Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 481380..482017 Replicon chromosome
Accession NZ_CP076514
Organism Bacillus velezensis strain WB

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KNV92_RS02465 Protein ID WP_003156187.1
Coordinates 481667..482017 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag KNV92_RS02460 Protein ID WP_003156188.1
Coordinates 481380..481661 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNV92_RS02440 (KNV92_02440) 477745..478344 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
KNV92_RS02445 (KNV92_02445) 478437..478802 + 366 WP_070081400.1 holo-ACP synthase -
KNV92_RS02450 (KNV92_02450) 478967..479974 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
KNV92_RS02455 (KNV92_02455) 480091..481260 + 1170 WP_077721837.1 alanine racemase -
KNV92_RS02460 (KNV92_02460) 481380..481661 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KNV92_RS02465 (KNV92_02465) 481667..482017 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KNV92_RS02470 (KNV92_02470) 482135..482956 + 822 WP_003156182.1 STAS domain-containing protein -
KNV92_RS02475 (KNV92_02475) 482961..483326 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
KNV92_RS02480 (KNV92_02480) 483329..483730 + 402 WP_003156178.1 anti-sigma regulatory factor -
KNV92_RS02485 (KNV92_02485) 483742..484749 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
KNV92_RS02490 (KNV92_02490) 484813..485142 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
KNV92_RS02495 (KNV92_02495) 485139..485621 + 483 WP_007609591.1 anti-sigma B factor RsbW -
KNV92_RS02500 (KNV92_02500) 485587..486375 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
KNV92_RS02505 (KNV92_02505) 486375..486977 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T205882 WP_003156187.1 NZ_CP076514:481667-482017 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T205882 NZ_CP101921:2716286-2716393 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT205882 WP_003156188.1 NZ_CP076514:481380-481661 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT205882 NZ_CP101921:c2716239-2716173 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References