Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 81199..81733 Replicon plasmid pUAMSEL1
Accession NZ_CP076499
Organism Enterococcus faecalis strain UAMS_EL53

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag KPU58_RS15415 Protein ID WP_002360769.1
Coordinates 81458..81733 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag KPU58_RS15410 Protein ID WP_002369771.1
Coordinates 81199..81465 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPU58_RS15380 (77302) 77302..78087 + 786 WP_000803546.1 winged helix-turn-helix domain-containing protein -
KPU58_RS15385 (78093) 78093..78581 + 489 WP_002368088.1 hypothetical protein -
KPU58_RS15390 (79173) 79173..79439 + 267 WP_002368091.1 hypothetical protein -
KPU58_RS15395 (79472) 79472..79966 + 495 WP_002368092.1 DnaJ domain-containing protein -
KPU58_RS15400 (80064) 80064..80480 + 417 WP_002368093.1 single-stranded DNA-binding protein -
KPU58_RS15405 (80504) 80504..81088 + 585 WP_002368095.1 thermonuclease family protein -
KPU58_RS15410 (81199) 81199..81465 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
KPU58_RS15415 (81458) 81458..81733 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
KPU58_RS15420 (82536) 82536..82721 + 186 WP_002368099.1 hypothetical protein -
KPU58_RS15425 (82743) 82743..82889 + 147 WP_002368101.1 DpnD/PcfM family protein -
KPU58_RS15430 (83120) 83120..83899 + 780 WP_002368102.1 Cas9 inhibitor AcrIIA9 family protein -
KPU58_RS15435 (83899) 83899..85158 + 1260 WP_002368103.1 PcfJ domain-containing protein -
KPU58_RS15440 (85808) 85808..86536 + 729 WP_002368105.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / ant(6)-Ia / aph(3')-III cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI 1..119720 119720


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T205877 WP_002360769.1 NZ_CP076499:81458-81733 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T205877 NZ_CP101921:1861595-1861849 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT205877 WP_002369771.1 NZ_CP076499:81199-81465 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT205877 NZ_CP101921:1861347-1861598 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References