Detailed information of TA system TA205811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_26(antitoxin)
• Location: 364659..365357
• Replicon: chromosome
• Accession: NZ_CP076493
• Organism: Enterococcus faecalis strain UAMS_EL54

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A059N529
• Locus tag: KPU55_RS01755
• Protein ID: WP_002373917.1
• Coordinates: 364659..365003 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: KPU55_RS01760
• Protein ID: WP_002388206.1
• Coordinates: 365022..365357 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPU55_RS01735 (361895)	361895..362044	+	150	WP_002356321.1	50S ribosomal protein L33	-
KPU55_RS01740 (362147)	362147..363328	-	1182	WP_002365138.1	site-specific integrase	-
KPU55_RS01745 (363483)	363483..364214	-	732	WP_010706861.1	potassium channel family protein	-
KPU55_RS01750 (364232)	364232..364630	-	399	WP_010706860.1	hypothetical protein	-
KPU55_RS01755 (364659)	364659..365003	-	345	WP_002373917.1	ImmA/IrrE family metallo-endopeptidase	Toxin
KPU55_RS01760 (365022)	365022..365357	-	336	WP_002388206.1	helix-turn-helix transcriptional regulator	Antitoxin
KPU55_RS01765 (365649)	365649..365831	+	183	WP_002388205.1	hypothetical protein	-
KPU55_RS01770 (365844)	365844..366182	+	339	WP_010706859.1	hypothetical protein	-
KPU55_RS01775 (366245)	366245..366424	+	180	WP_229202013.1	DUF2829 domain-containing protein	-
KPU55_RS01780 (366442)	366442..366711	+	270	WP_002365131.1	hypothetical protein	-
KPU55_RS01785 (366746)	366746..366916	+	171	WP_010706857.1	hypothetical protein	-
KPU55_RS01790 (366917)	366917..367333	+	417	WP_002417286.1	hypothetical protein	-
KPU55_RS01795 (367420)	367420..367719	+	300	WP_010706856.1	hypothetical protein	-
KPU55_RS01800 (367716)	367716..367940	+	225	WP_002367281.1	hypothetical protein	-
KPU55_RS01805 (367943)	367943..368299	+	357	WP_010706855.1	hypothetical protein	-
KPU55_RS01810 (368392)	368392..369363	+	972	WP_010706854.1	RecT family recombinase	-
KPU55_RS01815 (369326)	369326..370141	+	816	WP_010706853.1	PD-(D/E)XK nuclease-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-60)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13594.61 Da
• Isoelectric Point: 5.6177

>T205811 WP_002373917.1 NZ_CP076493:c365003-364659 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMALYKIPVFRSKMEAEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T205811 NZ_CP101911:c5292682-5292404 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12842.51 Da
• Isoelectric Point: 5.2926

>AT205811 WP_002388206.1 NZ_CP076493:c365357-365022 [Enterococcus faecalis]
MTVFDRIKLLAKERGKSLNRIEEELGLPKNVLYRMKTSDNPTKDRLETLANYFDVSVDYLLGRTDNTKATDEKKSDDLDD
VLDNVMSFDGKPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 336 bp

>AT205811 NZ_CP101911:c5292392-5292087 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A059N529