Detailed information of TA system TA020580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2124215..2124744
• Replicon: chromosome
• Accession: NC_002951
• Organism: Staphylococcus aureus subsp. aureus COL

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SACOL_RS10775
• Protein ID: WP_000621175.1
• Coordinates: 2124215..2124577 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SACOL_RS10780
• Protein ID: WP_000948331.1
• Coordinates: 2124574..2124744 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACOL_RS10750	2121192..2121962	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
SACOL_RS10755	2121937..2122416	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SACOL_RS10760	2122418..2122744	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SACOL_RS10765	2122864..2123865	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SACOL_RS10775	2124215..2124577	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SACOL_RS10780	2124574..2124744	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SACOL_RS10785	2124829..2125977	-	1149	WP_001281145.1	alanine racemase	-
SACOL_RS10790	2126043..2126402	-	360	WP_000581200.1	holo-ACP synthase	-
SACOL_RS10795	2126406..2126897	-	492	WP_001205910.1	PH domain-containing protein	-
SACOL_RS10800	2126884..2128467	-	1584	WP_001294626.1	PH domain-containing protein	-
SACOL_RS10805	2128460..2128939	-	480	WP_001287088.1	hypothetical protein	-
SACOL_RS10810	2129148..2129708	-	561	WP_001092407.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T20580 WP_000621175.1 NC_002951:c2124577-2124215 [Staphylococcus aureus subsp. aureus COL]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T20580 NC_002951:c2124577-2124215 [Staphylococcus aureus subsp. aureus COL]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT20580 WP_000948331.1 NC_002951:c2124744-2124574 [Staphylococcus aureus subsp. aureus COL]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT20580 NC_002951:c2124744-2124574 [Staphylococcus aureus subsp. aureus COL]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1