Detailed information of TA system TA020578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 908472..909004
• Replicon: chromosome
• Accession: NC_002951
• Organism: Staphylococcus aureus subsp. aureus COL

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: Q93CD4
• Locus tag: SACOL_RS04595
• Protein ID: WP_001103942.1
• Coordinates: 908684..909004 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: SACOL_RS04590
• Protein ID: WP_001058486.1
• Coordinates: 908472..908681 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACOL_RS04545	903491..904711	-	1221	WP_000266157.1	site-specific integrase	-
SACOL_RS04550	904800..905528	-	729	WP_000733775.1	staphylococcal enterotoxin type K	-
SACOL_RS04555	905552..906280	-	729	WP_001033316.1	staphylococcal enterotoxin type Q	-
SACOL_RS04560	906350..906781	-	432	WP_001228759.1	hypothetical protein	-
SACOL_RS04565	906798..907256	-	459	WP_000281657.1	ImmA/IrrE family metallo-endopeptidase	-
SACOL_RS04570	907268..907600	-	333	WP_001260004.1	helix-turn-helix transcriptional regulator	-
SACOL_RS04575	907793..908056	+	264	WP_000243851.1	helix-turn-helix domain-containing protein	-
SACOL_RS04580	908049..908321	+	273	WP_000091731.1	helix-turn-helix domain-containing protein	-
SACOL_RS04585	908333..908479	+	147	WP_000784875.1	hypothetical protein	-
SACOL_RS04590	908472..908681	+	210	WP_001058486.1	hypothetical protein	Antitoxin
SACOL_RS04595	908684..909004	+	321	WP_001103942.1	DUF1474 family protein	Toxin
SACOL_RS04600	909068..909937	+	870	WP_001002732.1	primase alpha helix C-terminal domain-containing protein	-
SACOL_RS04605	909954..911411	+	1458	WP_000390455.1	virulence-associated E family protein	-
SACOL_RS04610	911712..912074	+	363	WP_001039168.1	hypothetical protein	-
SACOL_RS04615	912076..912360	+	285	WP_000998181.1	hypothetical protein	-
SACOL_RS04620	912357..912998	+	642	WP_001019764.1	hypothetical protein	-
SACOL_RS04625	913515..913856	+	342	WP_001190615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	selk / selq / seb	885542..918806	33264

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12694.22 Da
• Isoelectric Point: 4.7419

>T20578 WP_001103942.1 NC_002951:908684-909004 [Staphylococcus aureus subsp. aureus COL]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFSELIQKF
HEIEKASSENFDEESDDAKNSIKVAE

Nucleotide

• Length: 321 bp

>T20578 NC_002951:908684-909004 [Staphylococcus aureus subsp. aureus COL]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCAGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCGTCATCAGAGAACTTTGACGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGAGTA
A

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8252.31 Da
• Isoelectric Point: 4.3718

>AT20578 WP_001058486.1 NC_002951:908472-908681 [Staphylococcus aureus subsp. aureus COL]
MNNEQKEVIEHLVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT20578 NC_002951:908472-908681 [Staphylococcus aureus subsp. aureus COL]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: Q93CD4