Detailed information of TA system TA205759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 126169..126776
• Replicon: plasmid pUAMSEF1
• Accession: NZ_CP076484
• Organism: Enterococcus faecium strain UAMS_EF55

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: KPU59_RS14370
• Protein ID: WP_002321032.1
• Coordinates: 126169..126519 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: KPU59_RS14375
• Protein ID: WP_002287514.1
• Coordinates: 126513..126776 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPU59_RS14360 (KPU59_14325)	122446..124308	-	1863	WP_049048592.1	group II intron reverse transcriptase/maturase	-
KPU59_RS14365 (KPU59_14330)	125531..126145	+	615	WP_002318230.1	recombinase family protein	-
KPU59_RS14370 (KPU59_14335)	126169..126519	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KPU59_RS14375 (KPU59_14340)	126513..126776	-	264	WP_002287514.1	hypothetical protein	Antitoxin
KPU59_RS14380 (KPU59_14345)	127014..128159	-	1146	WP_002305757.1	IS200/IS605 family element RNA-guided endonuclease TnpB	-
KPU59_RS14385 (KPU59_14350)	128171..128572	-	402	WP_002305759.1	IS200/IS605 family transposase	-
KPU59_RS14390 (KPU59_14355)	128745..129011	-	267	Protein_132	hypothetical protein	-
KPU59_RS14395 (KPU59_14360)	128993..129409	-	417	WP_002305764.1	hypothetical protein	-
KPU59_RS14400 (KPU59_14365)	129425..129676	-	252	WP_002305766.1	hypothetical protein	-
KPU59_RS14405 (KPU59_14370)	129676..129882	-	207	WP_002296239.1	hypothetical protein	-
KPU59_RS14410 (KPU59_14375)	130327..130587	-	261	WP_002321303.1	hypothetical protein	-
KPU59_RS14415 (KPU59_14380)	130953..131426	-	474	WP_002321302.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..264192	264192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T205759 WP_002321032.1 NZ_CP076484:c126519-126169 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T205759 NZ_CP101877:787189-787599 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT205759 WP_002287514.1 NZ_CP076484:c126776-126513 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT205759 NZ_CP101877:786942-787208 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0