Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 545284..545855 Replicon chromosome
Accession NZ_CP076483
Organism Enterococcus faecium strain UAMS_EF55

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag KPU59_RS02555 Protein ID WP_002286801.1
Coordinates 545514..545855 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag KPU59_RS02550 Protein ID WP_002323011.1
Coordinates 545284..545514 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPU59_RS02525 (KPU59_02525) 540417..541748 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
KPU59_RS02530 (KPU59_02530) 541770..542396 + 627 WP_002286816.1 cysteine hydrolase -
KPU59_RS02535 (KPU59_02535) 542579..543160 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
KPU59_RS02540 543892..544454 + 563 Protein_506 SOS response-associated peptidase family protein -
KPU59_RS02545 (KPU59_02550) 544659..544997 - 339 WP_002286804.1 hypothetical protein -
KPU59_RS02550 (KPU59_02555) 545284..545514 + 231 WP_002323011.1 hypothetical protein Antitoxin
KPU59_RS02555 (KPU59_02560) 545514..545855 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KPU59_RS02560 (KPU59_02565) 546888..548075 - 1188 WP_002296840.1 IS256-like element IS16 family transposase -
KPU59_RS02565 (KPU59_02570) 548869..549063 + 195 WP_002297028.1 hypothetical protein -
KPU59_RS02570 (KPU59_02575) 549254..549503 + 250 Protein_512 transposase -
KPU59_RS02575 (KPU59_02580) 549747..550577 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 546888..548075 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T205758 WP_002286801.1 NZ_CP076483:545514-545855 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T205758 NZ_CP101877:354812-355159 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT205758 WP_002323011.1 NZ_CP076483:545284-545514 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT205758 NZ_CP101877:355159-355452 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References