Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 202325..202854 Replicon chromosome
Accession NZ_CP076483
Organism Enterococcus faecium strain UAMS_EF55

Toxin (Protein)


Gene name relE Uniprot ID A0A828ZPW6
Locus tag KPU59_RS00990 Protein ID WP_002301121.1
Coordinates 202588..202854 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C9B8W8
Locus tag KPU59_RS00985 Protein ID WP_002294199.1
Coordinates 202325..202588 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPU59_RS00975 (KPU59_00970) 199700..201523 + 1824 WP_002294202.1 APC family permease -
KPU59_RS00980 (KPU59_00975) 201665..201850 - 186 WP_002348885.1 hypothetical protein -
KPU59_RS00985 (KPU59_00980) 202325..202588 + 264 WP_002294199.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KPU59_RS00990 (KPU59_00985) 202588..202854 + 267 WP_002301121.1 Txe/YoeB family addiction module toxin Toxin
KPU59_RS00995 203055..203543 + 489 WP_002301120.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
KPU59_RS15955 203503..203625 + 123 WP_285894660.1 hypothetical protein -
KPU59_RS01000 (KPU59_00995) 204085..204756 - 672 WP_002301116.1 ABC transporter permease -
KPU59_RS01005 (KPU59_01000) 204753..205670 - 918 WP_002301114.1 osmoprotectant ABC transporter substrate-binding protein -
KPU59_RS01010 (KPU59_01005) 205667..206308 - 642 WP_002293405.1 ABC transporter permease -
KPU59_RS01015 (KPU59_01010) 206312..207487 - 1176 WP_002348889.1 betaine/proline/choline family ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - lap 92765..226988 134223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(22-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10619.04 Da        Isoelectric Point: 9.8346

>T205757 WP_002301121.1 NZ_CP076483:202588-202854 [Enterococcus faecium]
MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T205757 NZ_CP101877:328841-329209 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10016.03 Da        Isoelectric Point: 4.1207

>AT205757 WP_002294199.1 NZ_CP076483:202325-202588 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNID
DIDWEAL

Download         Length: 264 bp

>AT205757 NZ_CP101877:328623-328844 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZPW6

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZU84

References