Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 31839..32446 Replicon plasmid pUAMSEF3
Accession NZ_CP076480
Organism Enterococcus faecium strain UAMS_EF57

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag KPU56_RS15485 Protein ID WP_002321032.1
Coordinates 32096..32446 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag KPU56_RS15480 Protein ID WP_002287514.1
Coordinates 31839..32102 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPU56_RS15440 (KPU56_15405) 27189..27662 + 474 WP_002321302.1 single-stranded DNA-binding protein -
KPU56_RS15445 (KPU56_15410) 28028..28288 + 261 WP_002321303.1 hypothetical protein -
KPU56_RS15450 (KPU56_15415) 28733..28939 + 207 WP_002296239.1 hypothetical protein -
KPU56_RS15455 (KPU56_15420) 28939..29190 + 252 WP_002305766.1 hypothetical protein -
KPU56_RS15460 (KPU56_15425) 29206..29622 + 417 WP_002305764.1 hypothetical protein -
KPU56_RS15465 (KPU56_15430) 29604..29870 + 267 Protein_30 hypothetical protein -
KPU56_RS15470 (KPU56_15435) 30043..30444 + 402 WP_002305759.1 IS200/IS605 family transposase -
KPU56_RS15475 (KPU56_15440) 30456..31601 + 1146 WP_002305757.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
KPU56_RS15480 (KPU56_15445) 31839..32102 + 264 WP_002287514.1 hypothetical protein Antitoxin
KPU56_RS15485 (KPU56_15450) 32096..32446 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KPU56_RS15490 (KPU56_15455) 32470..33084 - 615 WP_002318230.1 recombinase family protein -
KPU56_RS15495 (KPU56_15460) 34307..36169 + 1863 WP_049048592.1 group II intron reverse transcriptase/maturase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..57509 57509


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T205756 WP_002321032.1 NZ_CP076480:32096-32446 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T205756 NZ_CP101869:7314-7592 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGT
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCCGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT205756 WP_002287514.1 NZ_CP076480:31839-32102 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT205756 NZ_CP101869:7045-7314 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References