Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 28643..29250 Replicon plasmid pUAMSEF3
Accession NZ_CP076474
Organism Enterococcus faecium strain UAMS_EF58

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag KPU57_RS15450 Protein ID WP_002321032.1
Coordinates 28643..28993 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag KPU57_RS15455 Protein ID WP_002287514.1
Coordinates 28987..29250 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPU57_RS15440 (KPU57_15415) 24920..26782 - 1863 WP_049048592.1 group II intron reverse transcriptase/maturase -
KPU57_RS15445 (KPU57_15420) 28005..28619 + 615 WP_002318230.1 recombinase family protein -
KPU57_RS15450 (KPU57_15425) 28643..28993 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KPU57_RS15455 (KPU57_15430) 28987..29250 - 264 WP_002287514.1 hypothetical protein Antitoxin
KPU57_RS15460 (KPU57_15435) 29488..30633 - 1146 WP_002305757.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
KPU57_RS15465 (KPU57_15440) 30645..31046 - 402 WP_002305759.1 IS200/IS605 family transposase -
KPU57_RS15470 (KPU57_15445) 31219..31485 - 267 Protein_33 hypothetical protein -
KPU57_RS15475 (KPU57_15450) 31467..31883 - 417 WP_002305764.1 hypothetical protein -
KPU57_RS15480 (KPU57_15455) 31899..32150 - 252 WP_002305766.1 hypothetical protein -
KPU57_RS15485 (KPU57_15460) 32150..32356 - 207 WP_002296239.1 hypothetical protein -
KPU57_RS15490 (KPU57_15465) 32801..33061 - 261 WP_002321303.1 hypothetical protein -
KPU57_RS15495 (KPU57_15470) 33427..33900 - 474 WP_002321302.1 single-stranded DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..57509 57509


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T205752 WP_002321032.1 NZ_CP076474:c28993-28643 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T205752 NZ_CP101866:4018764-4019114 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT205752 WP_002287514.1 NZ_CP076474:c29250-28987 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT205752 NZ_CP101866:4018519-4018770 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References