Detailed information of TA system TA205748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4235083..4235721
• Replicon: chromosome
• Accession: NZ_CP076470
• Organism: Escherichia coli strain DH5a

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KN807_RS20460
• Protein ID: WP_000813794.1
• Coordinates: 4235545..4235721 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KN807_RS20455
• Protein ID: WP_001270286.1
• Coordinates: 4235083..4235499 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN807_RS20435 (4230235)	4230235..4231176	-	942	WP_001251304.1	ABC transporter permease	-
KN807_RS20440 (4231177)	4231177..4232190	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KN807_RS20445 (4232208)	4232208..4233353	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KN807_RS20450 (4233598)	4233598..4235004	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KN807_RS20455 (4235083)	4235083..4235499	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KN807_RS20460 (4235545)	4235545..4235721	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KN807_RS20465 (4235943)	4235943..4236173	+	231	WP_000494244.1	YncJ family protein	-
KN807_RS20470 (4236265)	4236265..4238226	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KN807_RS20475 (4238299)	4238299..4238835	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KN807_RS20480 (4238927)	4238927..4240102	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T205748 WP_000813794.1 NZ_CP076470:c4235721-4235545 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T205748 NZ_CP101866:3622125-3622403 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT205748 WP_001270286.1 NZ_CP076470:c4235499-4235083 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT205748 NZ_CP101866:3621862-3622122 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCTTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 6HPB