Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3666481..3667312 Replicon chromosome
Accession NZ_CP076470
Organism Escherichia coli strain DH5a

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag KN807_RS17560 Protein ID WP_000854814.1
Coordinates 3666481..3666855 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag KN807_RS17565 Protein ID WP_001285584.1
Coordinates 3666944..3667312 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KN807_RS17520 (3661877) 3661877..3663043 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
KN807_RS17525 (3663162) 3663162..3663635 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
KN807_RS17530 (3663833) 3663833..3664891 + 1059 WP_046613327.1 FUSC family protein -
KN807_RS17535 (3665063) 3665063..3665392 + 330 WP_000450409.1 DUF496 family protein -
KN807_RS17540 (3665493) 3665493..3665627 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
KN807_RS17545 (3665747) 3665747..3665875 + 129 Protein_3426 transposase domain-containing protein -
KN807_RS17550 (3666164) 3666164..3666244 - 81 Protein_3427 hypothetical protein -
KN807_RS17555 (3666290) 3666290..3666484 - 195 WP_000988600.1 DUF5983 family protein -
KN807_RS17560 (3666481) 3666481..3666855 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KN807_RS17565 (3666944) 3666944..3667312 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
KN807_RS17570 (3667386) 3667386..3667607 - 222 WP_000692323.1 DUF987 domain-containing protein -
KN807_RS17575 (3667670) 3667670..3668116 - 447 WP_000187523.1 RadC family protein -
KN807_RS17580 (3668113) 3668113..3669645 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T205737 WP_000854814.1 NZ_CP076470:c3666855-3666481 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T205737 NZ_CP101866:993545-993880 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAAGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCTTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACACTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT205737 WP_001285584.1 NZ_CP076470:c3667312-3666944 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT205737 NZ_CP101866:993297-993545 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATTCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGGCATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References