Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2710206..2710860 Replicon chromosome
Accession NZ_CP076470
Organism Escherichia coli strain DH5a

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KN807_RS13105 Protein ID WP_000244777.1
Coordinates 2710453..2710860 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KN807_RS13100 Protein ID WP_000354046.1
Coordinates 2710206..2710472 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KN807_RS13075 (2705375) 2705375..2706118 + 744 WP_000951964.1 SDR family oxidoreductase -
KN807_RS13080 (2706175) 2706175..2707608 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KN807_RS13085 (2707653) 2707653..2707964 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KN807_RS13090 (2708128) 2708128..2708787 + 660 WP_000250274.1 hemolysin III family protein -
KN807_RS13095 (2708983) 2708983..2709963 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KN807_RS13100 (2710206) 2710206..2710472 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KN807_RS13105 (2710453) 2710453..2710860 + 408 WP_000244777.1 protein YgfX Toxin
KN807_RS13110 (2710900) 2710900..2711421 - 522 WP_001055874.1 flavodoxin FldB -
KN807_RS13115 (2711533) 2711533..2712429 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KN807_RS13120 (2712454) 2712454..2713164 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KN807_RS13125 (2713170) 2713170..2714903 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T205732 WP_000244777.1 NZ_CP076470:2710453-2710860 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205732 NZ_CP101861:20272-20523 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205732 WP_000354046.1 NZ_CP076470:2710206-2710472 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205732 NZ_CP101861:20510-20737 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References