Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1398476..1398973 Replicon chromosome
Accession NZ_CP076470
Organism Escherichia coli strain DH5a

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KN807_RS06870 Protein ID WP_001173343.1
Coordinates 1398476..1398649 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KN807_RS06875 Protein ID WP_000398619.1
Coordinates 1398677..1398973 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KN807_RS06860 (1395146) 1395146..1396603 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KN807_RS06865 (1396840) 1396840..1398357 + 1518 WP_001295074.1 lysine--tRNA ligase -
KN807_RS06870 (1398476) 1398476..1398649 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KN807_RS06875 (1398677) 1398677..1398973 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KN807_RS06880 (1399200) 1399200..1399472 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KN807_RS06885 (1399484) 1399484..1399714 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KN807_RS06890 (1399895) 1399895..1401526 + 1632 WP_001216456.1 sensor histidine kinase -
KN807_RS06895 (1401523) 1401523..1402242 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T205726 WP_001173343.1 NZ_CP076470:c1398649-1398476 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T205726 NZ_CP101858:c4167022-4166744 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT205726 WP_000398619.1 NZ_CP076470:c1398973-1398677 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT205726 NZ_CP101858:c4167285-4167025 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References