Detailed information of TA system TA205724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1217504..1218318
• Replicon: chromosome
• Accession: NZ_CP076470
• Organism: Escherichia coli strain DH5a

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: KN807_RS05945
• Protein ID: WP_001054376.1
• Coordinates: 1217504..1217761 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: KN807_RS05950
• Protein ID: WP_001309181.1
• Coordinates: 1217773..1218318 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN807_RS05920 (1212792)	1212792..1213898	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
KN807_RS05925 (1213963)	1213963..1214943	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
KN807_RS05930 (1215053)	1215053..1215258	+	206	Protein_1159	HNH endonuclease	-
KN807_RS05935 (1215526)	1215526..1216766	-	1241	Protein_1160	helicase YjhR	-
KN807_RS05940 (1216882)	1216882..1217013	+	132	WP_001309182.1	hypothetical protein	-
KN807_RS05945 (1217504)	1217504..1217761	+	258	WP_001054376.1	YjhX family toxin	Toxin
KN807_RS05950 (1217773)	1217773..1218318	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
KN807_RS05955 (1218374)	1218374..1219120	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
KN807_RS05960 (1219289)	1219289..1219507	+	219	Protein_1165	hypothetical protein	-
KN807_RS05965 (1219545)	1219545..1219661	+	117	Protein_1166	VOC family protein	-
KN807_RS05970 (1219906)	1219906..1221027	+	1122	WP_000010829.1	M42 family metallopeptidase	-
KN807_RS05975 (1221024)	1221024..1221302	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
KN807_RS05980 (1221314)	1221314..1222627	+	1314	WP_000460842.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	1206514..1226542	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T205724 WP_001054376.1 NZ_CP076470:1217504-1217761 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T205724 NZ_CP101858:3535548-3535721 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT205724 WP_001309181.1 NZ_CP076470:1217773-1218318 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT205724 NZ_CP101858:3535224-3535520 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8