Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 845893..846572 | Replicon | chromosome |
| Accession | NZ_CP076470 | ||
| Organism | Escherichia coli strain DH5a | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | KN807_RS04180 | Protein ID | WP_000854672.1 |
| Coordinates | 846231..846572 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | KN807_RS04175 | Protein ID | WP_000070395.1 |
| Coordinates | 845893..846210 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KN807_RS04125 (840940) | 840940..841803 | + | 864 | WP_001065553.1 | GTPase family protein | - |
| KN807_RS04130 (841895) | 841895..842716 | + | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| KN807_RS04135 (842933) | 842933..843124 | + | 192 | Protein_809 | DeoR family transcriptional regulator | - |
| KN807_RS04140 (843120) | 843120..843347 | + | 228 | WP_001548158.1 | protein YpjK | - |
| KN807_RS04145 (843347) | 843347..843790 | + | 444 | WP_000824223.1 | lipoprotein YafY | - |
| KN807_RS04150 (843813) | 843813..844280 | + | 468 | WP_001547765.1 | protein YkfB | - |
| KN807_RS04155 (844357) | 844357..844596 | + | 240 | WP_000194654.1 | DUF905 family protein | - |
| KN807_RS04160 (844694) | 844694..845152 | + | 459 | WP_000211838.1 | antirestriction protein | - |
| KN807_RS04165 (845168) | 845168..845644 | + | 477 | WP_000811693.1 | RadC family protein | - |
| KN807_RS04170 (845653) | 845653..845874 | + | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| KN807_RS04175 (845893) | 845893..846210 | + | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| KN807_RS04180 (846231) | 846231..846572 | + | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| KN807_RS04190 (847144) | 847144..848397 | - | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| KN807_RS04195 (848409) | 848409..849512 | - | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| KN807_RS04200 (849800) | 849800..850159 | + | 360 | Protein_821 | porin | - |
| KN807_RS04205 (850266) | 850266..850364 | + | 99 | Protein_822 | hypothetical protein | - |
| KN807_RS04210 (850363) | 850363..850842 | + | 480 | Protein_823 | porin | - |
| KN807_RS04215 (850998) | 850998..851300 | - | 303 | Protein_824 | sigma factor-binding protein Crl | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T205719 WP_000854672.1 NZ_CP076470:846231-846572 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T205719 NZ_CP101858:c2151990-2151583 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT205719 WP_000070395.1 NZ_CP076470:845893-846210 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT205719 NZ_CP101858:c2152237-2151971 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTAATCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTAATCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|