Detailed information of TA system TA205718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 727155..727773
• Replicon: chromosome
• Accession: NZ_CP076470
• Organism: Escherichia coli strain DH5a

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: KN807_RS03545
• Protein ID: WP_001290581.1
• Coordinates: 727555..727773 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: KN807_RS03540
• Protein ID: WP_000344800.1
• Coordinates: 727155..727529 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN807_RS03530 (722244)	722244..723437	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
KN807_RS03535 (723460)	723460..726609	+	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
KN807_RS03540 (727155)	727155..727529	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
KN807_RS03545 (727555)	727555..727773	+	219	WP_001290581.1	HHA domain-containing protein	Toxin
KN807_RS03550 (727945)	727945..728496	+	552	WP_000102564.1	maltose O-acetyltransferase	-
KN807_RS03555 (728612)	728612..729082	+	471	WP_000136192.1	YlaC family protein	-
KN807_RS03560 (729246)	729246..730796	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
KN807_RS03565 (730838)	730838..731191	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
KN807_RS03575 (731570)	731570..731881	+	312	WP_000409911.1	MGMT family protein	-
KN807_RS03580 (731912)	731912..732484	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T205718 WP_001290581.1 NZ_CP076470:727555-727773 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T205718 NZ_CP101858:c2015807-2015472 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT205718 WP_000344800.1 NZ_CP076470:727155-727529 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT205718 NZ_CP101858:c2016055-2015807 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGAGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5