Detailed information of TA system TA205716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 32094..32629
• Replicon: plasmid pPM27_2
• Accession: NZ_CP076455
• Organism: Klebsiella pneumoniae strain PM27

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KOM10_RS27175
• Protein ID: WP_016531292.1
• Coordinates: 32094..32381 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W9BAK2
• Locus tag: KOM10_RS27180
• Protein ID: WP_016531291.1
• Coordinates: 32378..32629 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOM10_RS27155 (KOM10_27070)	27893..29113	-	1221	WP_032446666.1	ISL3-like element ISKox3 family transposase	-
KOM10_RS27160 (KOM10_27075)	29274..29750	+	477	WP_016529487.1	hypothetical protein	-
KOM10_RS27165 (KOM10_27080)	30007..30294	-	288	WP_016529486.1	hypothetical protein	-
KOM10_RS27170 (KOM10_27085)	30384..31046	-	663	WP_016529485.1	division plane positioning ATPase MipZ	-
KOM10_RS27175 (KOM10_27090)	32094..32381	-	288	WP_016531292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KOM10_RS27180 (KOM10_27095)	32378..32629	-	252	WP_016531291.1	toxin-antitoxin stability system antitoxin protein StbD	Antitoxin
KOM10_RS27185 (KOM10_27100)	32725..33996	-	1272	WP_046041953.1	Y-family DNA polymerase	-
KOM10_RS27190 (KOM10_27105)	33996..34490	-	495	WP_290127643.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KOM10_RS27195 (KOM10_27110)	34476..35555	+	1080	WP_016531016.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	clbI / basG	1..92566	92566
	-	flank	IS/Tn	-	-	27893..29113	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11120.16 Da
• Isoelectric Point: 10.4373

>T205716 WP_016531292.1 NZ_CP076455:c32381-32094 [Klebsiella pneumoniae]
MTYTVKFREDALKEWNKLDKTIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDNQLIIAIVAVGK
RERSDVYTLASERMK

Nucleotide

• Length: 288 bp

>T205716 NZ_CP101858:c1163823-1163572 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9285.68 Da
• Isoelectric Point: 4.2257

>AT205716 WP_016531291.1 NZ_CP076455:c32629-32378 [Klebsiella pneumoniae]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELIKLVAERSNQPLFDMDLE
KYL

Nucleotide

• Length: 252 bp

>AT205716 NZ_CP101858:c1163575-1163321 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: W9BAK2