Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16104..16747 Replicon plasmid pPM27_2
Accession NZ_CP076455
Organism Klebsiella pneumoniae strain PM27

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag KOM10_RS27095 Protein ID WP_001044768.1
Coordinates 16104..16520 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag KOM10_RS27100 Protein ID WP_001261287.1
Coordinates 16517..16747 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOM10_RS27060 (KOM10_26975) 11198..11452 - 255 WP_016528790.1 hypothetical protein -
KOM10_RS27065 (KOM10_26980) 11528..11785 - 258 WP_004098928.1 hypothetical protein -
KOM10_RS27070 (KOM10_26985) 11834..12037 - 204 WP_004150739.1 HHA domain-containing protein -
KOM10_RS27075 (KOM10_26990) 12098..12592 - 495 WP_016528789.1 hypothetical protein -
KOM10_RS27080 (KOM10_26995) 12623..13189 - 567 WP_009654312.1 hypothetical protein -
KOM10_RS27085 (KOM10_27000) 13186..13449 - 264 WP_009310051.1 hypothetical protein -
KOM10_RS27090 (KOM10_27005) 13804..15942 + 2139 WP_000350635.1 AAA family ATPase -
KOM10_RS27095 (KOM10_27010) 16104..16520 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
KOM10_RS27100 (KOM10_27015) 16517..16747 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KOM10_RS27105 (KOM10_27020) 17053..20172 + 3120 WP_000467135.1 hypothetical protein -
KOM10_RS27110 (KOM10_27025) 20435..21568 + 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - clbI / basG 1..92566 92566


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T205715 WP_001044768.1 NZ_CP076455:c16520-16104 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T205715 NZ_CP101858:c1163575-1163321 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT205715 WP_001261287.1 NZ_CP076455:c16747-16517 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT205715 NZ_CP101858:c1163823-1163572 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References