Detailed information of TA system TA205714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 97673..98424
• Replicon: plasmid pPM27_Vir
• Accession: NZ_CP076454
• Organism: Klebsiella pneumoniae strain PM27

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: W9BA31
• Locus tag: KOM10_RS26650
• Protein ID: WP_032104592.1
• Coordinates: 97673..98155 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: W9B1S8
• Locus tag: KOM10_RS26655
• Protein ID: WP_016529519.1
• Coordinates: 98146..98424 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOM10_RS26620 (KOM10_26545)	92990..94189	-	1200	WP_049202907.1	GTP-binding protein	-
KOM10_RS26625 (KOM10_26550)	94287..94739	+	453	WP_071829949.1	transcriptional repressor	-
KOM10_RS26630 (KOM10_26555)	94886..95602	+	717	WP_252919389.1	DDE-type integrase/transposase/recombinase	-
KOM10_RS26635 (KOM10_26560)	95987..96223	+	237	WP_016529523.1	hypothetical protein	-
KOM10_RS26640 (KOM10_26565)	96262..96894	-	633	WP_016529522.1	hypothetical protein	-
KOM10_RS26645 (KOM10_26570)	97231..97635	-	405	WP_016529521.1	DUF2251 domain-containing protein	-
KOM10_RS26650 (KOM10_26575)	97673..98155	-	483	WP_032104592.1	GNAT family N-acetyltransferase	Toxin
KOM10_RS26655 (KOM10_26580)	98146..98424	-	279	WP_016529519.1	DUF1778 domain-containing protein	Antitoxin
KOM10_RS26660 (KOM10_26585)	98732..99268	+	537	WP_032445791.1	hypothetical protein	-
KOM10_RS26665 (KOM10_26590)	99652..100662	-	1011	WP_004152284.1	zinc-binding alcohol dehydrogenase family protein	-
KOM10_RS26670	101005..101087	+	83	Protein_113	hypothetical protein	-
KOM10_RS26675 (KOM10_26595)	101123..102205	+	1083	WP_016528990.1	DNA-binding transcriptional repressor LacI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA / iroN / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..162330	162330
	-	flank	IS/Tn	-	-	94886..95602	716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17781.73 Da
• Isoelectric Point: 9.4946

>T205714 WP_032104592.1 NZ_CP076454:c98155-97673 [Klebsiella pneumoniae]
MGMRPPEPLTPEHNIAEFCCQDQVLSEWLKKKALKNHRTGISRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTEHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Nucleotide

• Length: 483 bp

>T205714 NZ_CP101858:1150400-1150774 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10708.23 Da
• Isoelectric Point: 4.5156

>AT205714 WP_016529519.1 NZ_CP076454:c98424-98146 [Klebsiella pneumoniae]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDNQFNAFVEELDAPMTPERQAR
IDNLMSRKSPWE

Nucleotide

• Length: 279 bp

>AT205714 NZ_CP101858:1149943-1150311 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: W9BA31

Antitoxin

• AlphaFold DB: W9B1S8