Detailed information of TA system TA205713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 27830..28740
• Replicon: plasmid pPM27_Vir
• Accession: NZ_CP076454
• Organism: Klebsiella pneumoniae strain PM27

Toxin (Protein)

• Gene name: -
• Uniprot ID: W9B9V2
• Locus tag: KOM10_RS26245
• Protein ID: WP_016528981.1
• Coordinates: 27830..28300 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W9B1L8
• Locus tag: KOM10_RS26250
• Protein ID: WP_016528982.1
• Coordinates: 28297..28740 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOM10_RS26215 (KOM10_26155)	22938..23144	-	207	Protein_22	IS66 family transposase	-
KOM10_RS26220 (KOM10_26160)	23629..23787	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
KOM10_RS26225 (KOM10_26165)	24428..24595	-	168	WP_004117900.1	hypothetical protein	-
KOM10_RS26230 (KOM10_26170)	25081..25860	+	780	WP_225932725.1	Hok/Gef family protein	-
KOM10_RS26240 (KOM10_26180)	27459..27719	+	261	WP_016528980.1	hypothetical protein	-
KOM10_RS26245 (KOM10_26185)	27830..28300	-	471	WP_016528981.1	RES family NAD+ phosphorylase	Toxin
KOM10_RS26250 (KOM10_26190)	28297..28740	-	444	WP_016528982.1	DUF2384 domain-containing protein	Antitoxin
KOM10_RS26255 (KOM10_26195)	28841..29330	-	490	Protein_30	DUF4113 domain-containing protein	-
KOM10_RS26260 (KOM10_26200)	29439..29660	-	222	WP_016528984.1	DUF2188 domain-containing protein	-
KOM10_RS26265 (KOM10_26205)	29835..29960	-	126	Protein_32	Fe3+-citrate ABC transporter substrate-binding protein	-
KOM10_RS26270 (KOM10_26210)	30035..32161	-	2127	WP_032105434.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
KOM10_RS26275 (KOM10_26215)	32248..33201	-	954	Protein_34	ferric citrate uptake sigma factor regulator FecR	-
KOM10_RS26280 (KOM10_26220)	33198..33719	-	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	rmpA / iroN / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..162330	162330

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17603.85 Da
• Isoelectric Point: 4.4093

>T205713 WP_016528981.1 NZ_CP076454:c28300-27830 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTWSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T205713 NZ_CP101858:c955975-955872 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16494.83 Da
• Isoelectric Point: 10.2498

>AT205713 WP_016528982.1 NZ_CP076454:c28740-28297 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEIIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT205713 NZ_CP101858:955834-956105 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: W9B9V2

Antitoxin

• AlphaFold DB: W9B1L8