Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4710091..4710901 Replicon chromosome
Accession NZ_CP076453
Organism Klebsiella pneumoniae strain PM27

Toxin (Protein)


Gene name KacT Uniprot ID W9BJB1
Locus tag KOM10_RS22770 Protein ID WP_016529513.1
Coordinates 4710091..4710624 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KOM10_RS22775 Protein ID WP_002887278.1
Coordinates 4710635..4710901 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOM10_RS22765 (KOM10_22705) 4708922..4710043 + 1122 WP_252919198.1 cupin domain-containing protein -
KOM10_RS22770 (KOM10_22710) 4710091..4710624 - 534 WP_016529513.1 type II toxin-antitoxin system toxin KacT Toxin
KOM10_RS22775 (KOM10_22715) 4710635..4710901 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KOM10_RS22780 (KOM10_22720) 4711004..4712437 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
KOM10_RS22785 (KOM10_22725) 4712427..4713110 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KOM10_RS22790 (KOM10_22730) 4713282..4714667 + 1386 WP_016530864.1 efflux transporter outer membrane subunit -
KOM10_RS22795 (KOM10_22735) 4714685..4715029 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19776.63 Da        Isoelectric Point: 5.2614

>T205709 WP_016529513.1 NZ_CP076453:c4710624-4710091 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGLDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T205709 NZ_CP101858:c322900-322793 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGACGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT205709 WP_002887278.1 NZ_CP076453:c4710901-4710635 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT205709 NZ_CP101858:322947-323013 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BJB1

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References