Detailed information of TA system TA205695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 332032..332618
• Replicon: chromosome
• Accession: NZ_CP076453
• Organism: Klebsiella pneumoniae strain PM27

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W9BMV7
• Locus tag: KOM10_RS01545
• Protein ID: WP_004185990.1
• Coordinates: 332250..332618 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: KOM10_RS01540
• Protein ID: WP_004174006.1
• Coordinates: 332032..332253 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOM10_RS01520 (KOM10_01510)	328189..329115	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KOM10_RS01525 (KOM10_01515)	329112..330389	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
KOM10_RS01530 (KOM10_01520)	330386..331153	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KOM10_RS01535 (KOM10_01525)	331155..331868	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KOM10_RS01540 (KOM10_01530)	332032..332253	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KOM10_RS01545 (KOM10_01535)	332250..332618	+	369	WP_004185990.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KOM10_RS01550 (KOM10_01540)	332891..334207	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KOM10_RS01555 (KOM10_01545)	334314..335201	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KOM10_RS01560 (KOM10_01550)	335198..336043	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KOM10_RS01565 (KOM10_01555)	336045..337115	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	292740..337852	45112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13469.85 Da
• Isoelectric Point: 8.0667

>T205695 WP_004185990.1 NZ_CP076453:332250-332618 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVCRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T205695 NZ_CP101844:277150-277407 [Streptococcus suis]
ATGGGAATCCATTTTACAGAGGATGCCTGGGAAGACTATCTTTATTGGCAAGGTCAGGATAAGAAAACACTCAAGCGAAT
CAATAAGATTATCAAGGATATGCAACGACATCCTTTTGAAGGCATTGCCAAACCAGAACCCTTGAAGTATGATTATCAGG
GTGCCTGGTCTCGGAGAATTGATGCCGAAAATCGGCTGATTTATATGGTAGAAGTAGATAAGCTGTATATTTTGTCTTTA
AAGGATCATTATGGGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT205695 WP_004174006.1 NZ_CP076453:332032-332253 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT205695 NZ_CP101844:276888-277148 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCAAAGAACGA
AACCTGATAGAGGCGGACTAA

Structures

Toxin

• AlphaFold DB: W9BMV7

Antitoxin

• AlphaFold DB: A0A5E5YJY7