Detailed information of TA system TA205690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1496281..1496918
• Replicon: chromosome
• Accession: NZ_CP076450
• Organism: Bacillus velezensis strain GMEKP1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KOM03_RS07580
• Protein ID: WP_003156187.1
• Coordinates: 1496568..1496918 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KOM03_RS07575
• Protein ID: WP_003156188.1
• Coordinates: 1496281..1496562 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOM03_RS07555 (KOM03_07555)	1492646..1493245	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
KOM03_RS07560 (KOM03_07560)	1493338..1493703	+	366	WP_007410229.1	holo-ACP synthase	-
KOM03_RS07565 (KOM03_07565)	1493868..1494875	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
KOM03_RS07570 (KOM03_07570)	1494992..1496161	+	1170	WP_025284272.1	alanine racemase	-
KOM03_RS07575 (KOM03_07575)	1496281..1496562	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KOM03_RS07580 (KOM03_07580)	1496568..1496918	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KOM03_RS07585 (KOM03_07585)	1497036..1497857	+	822	WP_003156182.1	STAS domain-containing protein	-
KOM03_RS07590 (KOM03_07590)	1497862..1498227	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
KOM03_RS07595 (KOM03_07595)	1498230..1498631	+	402	WP_003156178.1	anti-sigma regulatory factor	-
KOM03_RS07600 (KOM03_07600)	1498643..1499650	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
KOM03_RS07605 (KOM03_07605)	1499714..1500043	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
KOM03_RS07610 (KOM03_07610)	1500040..1500522	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
KOM03_RS07615 (KOM03_07615)	1500488..1501276	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
KOM03_RS07620 (KOM03_07620)	1501276..1501878	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T205690 WP_003156187.1 NZ_CP076450:1496568-1496918 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T205690 NZ_CP101835:c4787768-4787484 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT205690 WP_003156188.1 NZ_CP076450:1496281-1496562 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT205690 NZ_CP101835:c4788000-4787758 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5