Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/RHH(antitoxin)
Location 2846690..2847243 Replicon chromosome
Accession NC_002947
Organism Pseudomonas putida KT2440

Toxin (Protein)


Gene name parE Uniprot ID Q88JZ3
Locus tag PP_RS13055 Protein ID WP_010953434.1
Coordinates 2846950..2847243 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q88JZ4
Locus tag PP_RS13050 Protein ID WP_010953433.1
Coordinates 2846690..2846962 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PP_RS13020 (PP_2493) 2842262..2842621 - 360 Protein_2533 DNA repair protein RadC -
PP_RS13025 (PP_2494) 2842593..2843543 - 951 WP_031299400.1 hypothetical protein -
PP_RS13030 (PP_2495) 2843636..2844640 - 1005 WP_010953429.1 YqaJ viral recombinase family protein -
PP_RS13035 (PP_2496) 2844720..2845688 - 969 WP_031299399.1 DUF932 domain-containing protein -
PP_RS13040 (PP_2497) 2845786..2846058 - 273 WP_031299398.1 hypothetical protein -
PP_RS13045 (PP_2498) 2846320..2846565 + 246 WP_010953432.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
PP_RS13050 (PP_2499) 2846690..2846962 + 273 WP_010953433.1 CopG family ribbon-helix-helix protein Antitoxin
PP_RS13055 (PP_2500) 2846950..2847243 + 294 WP_010953434.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PP_RS13060 (PP_2501) 2847237..2848646 - 1410 WP_010953435.1 site-specific integrase -
PP_RS13065 (PP_2502) 2849169..2850095 - 927 WP_010953436.1 LysR family transcriptional regulator -
PP_RS13070 (PP_2503) 2850209..2850940 + 732 WP_010953437.1 SDR family oxidoreductase -
PP_RS13075 (PP_2504) 2851010..2851219 + 210 WP_010953438.1 4-oxalocrotonate tautomerase family protein -
PP_RS28390 (PP_5530) 2851216..2851428 + 213 WP_061405625.1 hypothetical protein -
PP_RS13085 (PP_5531) 2851571..2852089 + 519 WP_049587643.1 DUF1993 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-95)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-40)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11155.61 Da        Isoelectric Point: 5.9081

>T20569 WP_010953434.1 NC_002947:2846950-2847243 [Pseudomonas putida KT2440]
VPRLIVTEGAAKGLERCRRFLSDKDPQVARRAAQAIERQFARLEESPEVGRPFPDLPELRELIIEFGDSGYVALYRYERA
DDTAYVLAFRHQKEAGY

Download         Length: 294 bp

>T20569 NC_002947:2846950-2847243 [Pseudomonas putida KT2440]
GTGCCACGACTGATCGTCACTGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GGTCGCACGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCTTTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATCGAGTTTGGTGACTCAGGTTACGTCGCGCTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10373.53 Da        Isoelectric Point: 5.5744

>AT20569 WP_010953433.1 NC_002947:2846690-2846962 [Pseudomonas putida KT2440]
MATSIKIDDDLKGRIQHLACLRQRSSHWIMREAITQYVEREEARESFKQEALASWAAYQETGQHLTGQEARTWLGSWGTD
AEAELPKCHD

Download         Length: 273 bp

>AT20569 NC_002947:2846690-2846962 [Pseudomonas putida KT2440]
ATGGCCACTTCCATCAAAATTGACGACGATCTGAAAGGTCGCATTCAGCATTTGGCTTGTTTGCGCCAGCGCTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCATACCAGGAAACCGGCCAGCACTTGACCGGCCAGGAGGCTCGCACCTGGCTTGGTAGTTGGGGTACAGAT
GCTGAGGCAGAGTTACCCAAGTGCCACGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W5CNE8

Antitoxin

Source ID Structure
AlphaFold DB Q88JZ4

References