Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 1327843..1328759 | Replicon | chromosome |
| Accession | NZ_CP076445 | ||
| Organism | Bacillus subtilis subsp. subtilis strain ps4100 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | O34853 |
| Locus tag | KMZ16_RS06970 | Protein ID | WP_003244695.1 |
| Coordinates | 1328013..1328759 (-) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | G4EXD8 |
| Locus tag | KMZ16_RS06965 | Protein ID | WP_003232646.1 |
| Coordinates | 1327843..1328013 (-) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KMZ16_RS06930 (KMZ16_06885) | 1324709..1325035 | + | 327 | WP_069703538.1 | XkdW family protein | - |
| KMZ16_RS06935 (KMZ16_06890) | 1325032..1325196 | + | 165 | WP_014479563.1 | XkdX family protein | - |
| KMZ16_RS06940 (KMZ16_06895) | 1325240..1326079 | + | 840 | WP_029727045.1 | phage-like element PBSX protein XepA | - |
| KMZ16_RS06945 (KMZ16_06900) | 1326132..1326401 | + | 270 | WP_015252265.1 | hemolysin XhlA family protein | - |
| KMZ16_RS06950 (KMZ16_06905) | 1326414..1326677 | + | 264 | WP_014479566.1 | phage holin | - |
| KMZ16_RS06955 (KMZ16_06910) | 1326690..1327583 | + | 894 | WP_029727044.1 | N-acetylmuramoyl-L-alanine amidase | - |
| KMZ16_RS06960 (KMZ16_06915) | 1327620..1327757 | - | 138 | WP_119899277.1 | three component toxin-antitoxin-antitoxin system antitoxin SpoIISC | - |
| KMZ16_RS06965 (KMZ16_06920) | 1327843..1328013 | - | 171 | WP_003232646.1 | three component toxin-antitoxin-antitoxin system antitoxin SpoIISB | Antitoxin |
| KMZ16_RS06970 (KMZ16_06925) | 1328013..1328759 | - | 747 | WP_003244695.1 | toxin-antitoxin-antitoxin system toxin SpoIISA | Toxin |
| KMZ16_RS06975 (KMZ16_06930) | 1328869..1329870 | - | 1002 | WP_003232642.1 | inorganic phosphate transporter | - |
| KMZ16_RS06980 (KMZ16_06935) | 1329883..1330500 | - | 618 | WP_003218470.1 | DUF47 domain-containing protein | - |
| KMZ16_RS06985 (KMZ16_06940) | 1330776..1332092 | - | 1317 | WP_015252262.1 | serine/threonine exchanger | - |
| KMZ16_RS06990 (KMZ16_06945) | 1332481..1333431 | + | 951 | WP_014476561.1 | ring-cleaving dioxygenase | - |
| KMZ16_RS06995 | 1333540..1333635 | + | 96 | Protein_1314 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1292285..1338660 | 46375 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29061.50 Da Isoelectric Point: 4.6191
>T205680 WP_003244695.1 NZ_CP076445:c1328759-1328013 [Bacillus subtilis subsp. subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
Download Length: 747 bp
>T205680 NZ_CP076445:c1328759-1328013 [Bacillus subtilis subsp. subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTAGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAGCCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTAGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAGCCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6654.72 Da Isoelectric Point: 11.0203
>AT205680 WP_003232646.1 NZ_CP076445:c1328013-1327843 [Bacillus subtilis subsp. subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
Download Length: 171 bp
>AT205680 NZ_CP076445:c1328013-1327843 [Bacillus subtilis subsp. subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|