Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 516639..517275 | Replicon | chromosome |
| Accession | NZ_CP076445 | ||
| Organism | Bacillus subtilis subsp. subtilis strain ps4100 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | KMZ16_RS02635 | Protein ID | WP_003156187.1 |
| Coordinates | 516925..517275 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | KMZ16_RS02630 | Protein ID | WP_003225183.1 |
| Coordinates | 516639..516920 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KMZ16_RS02610 (KMZ16_02590) | 512998..513597 | - | 600 | WP_003246687.1 | rhomboid family intramembrane serine protease | - |
| KMZ16_RS02615 (KMZ16_02595) | 513692..514057 | + | 366 | WP_015252768.1 | holo-ACP synthase | - |
| KMZ16_RS02620 (KMZ16_02600) | 514223..515239 | + | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| KMZ16_RS02625 (KMZ16_02605) | 515354..516523 | + | 1170 | WP_003234284.1 | alanine racemase | - |
| KMZ16_RS02630 (KMZ16_02610) | 516639..516920 | + | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| KMZ16_RS02635 (KMZ16_02615) | 516925..517275 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KMZ16_RS02640 (KMZ16_02620) | 517390..518214 | + | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| KMZ16_RS02645 (KMZ16_02625) | 518219..518584 | + | 366 | WP_015715277.1 | RsbT antagonist protein RsbS | - |
| KMZ16_RS02650 (KMZ16_02630) | 518588..518989 | + | 402 | WP_003246640.1 | serine/threonine-protein kinase RsbT | - |
| KMZ16_RS02655 (KMZ16_02635) | 519001..520008 | + | 1008 | WP_003234295.1 | phosphoserine phosphatase RsbU | - |
| KMZ16_RS02660 (KMZ16_02640) | 520070..520399 | + | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| KMZ16_RS02665 (KMZ16_02645) | 520396..520878 | + | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| KMZ16_RS02670 (KMZ16_02650) | 520844..521632 | + | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| KMZ16_RS02675 (KMZ16_02655) | 521632..522231 | + | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T205679 WP_003156187.1 NZ_CP076445:516925-517275 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T205679 NZ_CP101831:c2712067-2711684 [Lacticaseibacillus paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAGGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAGGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT205679 WP_003225183.1 NZ_CP076445:516639-516920 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT205679 NZ_CP101831:c2712335-2712087 [Lacticaseibacillus paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCCGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCCGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|