205677

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1240998..1241915 Replicon chromosome
Accession NZ_CP076414
Organism Bacillus velezensis strain NJ13

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag KOM05_RS06500 Protein ID WP_007407256.1
Coordinates 1241169..1241915 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag KOM05_RS06495 Protein ID WP_003154807.1
Coordinates 1240998..1241168 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOM05_RS06455 (KOM05_06395) 1236231..1237853 + 1623 WP_032874601.1 pyocin knob domain-containing protein -
KOM05_RS06460 (KOM05_06400) 1237866..1238237 + 372 WP_032874603.1 XkdW family protein -
KOM05_RS06465 (KOM05_06405) 1238242..1238439 + 198 WP_032874605.1 XkdX family protein -
KOM05_RS06470 (KOM05_06410) 1238496..1239257 + 762 WP_032874607.1 hypothetical protein -
KOM05_RS06475 (KOM05_06415) 1239309..1239572 + 264 WP_003154815.1 hemolysin XhlA family protein -
KOM05_RS06480 (KOM05_06420) 1239586..1239849 + 264 WP_003154813.1 phage holin -
KOM05_RS06485 (KOM05_06425) 1239863..1240741 + 879 WP_032874609.1 N-acetylmuramoyl-L-alanine amidase -
KOM05_RS06490 1240776..1240901 - 126 WP_003154809.1 hypothetical protein -
KOM05_RS06495 (KOM05_06430) 1240998..1241168 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
KOM05_RS06500 (KOM05_06435) 1241169..1241915 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
KOM05_RS06505 (KOM05_06440) 1242020..1243018 - 999 WP_032874611.1 inorganic phosphate transporter -
KOM05_RS06510 (KOM05_06445) 1243031..1243648 - 618 WP_032874614.1 DUF47 domain-containing protein -
KOM05_RS06515 (KOM05_06450) 1243934..1245250 - 1317 WP_032874616.1 amino acid permease -
KOM05_RS06520 (KOM05_06455) 1245572..1246522 + 951 WP_032874618.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1180253..1299767 119514


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-239)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T205677 WP_007407256.1 NZ_CP076414:c1241915-1241169 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T205677 NZ_CP101831:660685-660966 [Lacticaseibacillus paracasei]
ATGAATAAATTTGTTTATAAACCAGCCTTTGAACGTCAATTTAAAAAACATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGATTTTGAAGCTGTTTATCATAAACTATTACGAGATGAACCGTTAGATCCGCATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAACTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT205677 WP_003154807.1 NZ_CP076414:c1241168-1240998 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT205677 NZ_CP101831:660450-660692 [Lacticaseibacillus paracasei]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAGTCGTGTGGGGTTAAC
TCCCGGGGAAGCTTTTCGTATCTTTGCCAAGAAATCCATTGAAGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGGAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGACTGGATTGGTTAAATGAA
TAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB I2HQ15


Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References