Detailed information of TA system TA205676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 489965..490602
• Replicon: chromosome
• Accession: NZ_CP076414
• Organism: Bacillus velezensis strain NJ13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KOM05_RS02510
• Protein ID: WP_003156187.1
• Coordinates: 490252..490602 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KOM05_RS02505
• Protein ID: WP_003156188.1
• Coordinates: 489965..490246 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOM05_RS02485 (KOM05_02460)	486330..486929	-	600	WP_032872997.1	rhomboid family intramembrane serine protease	-
KOM05_RS02490 (KOM05_02465)	487022..487387	+	366	WP_007609580.1	holo-ACP synthase	-
KOM05_RS02495 (KOM05_02470)	487552..488559	+	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
KOM05_RS02500 (KOM05_02475)	488676..489845	+	1170	WP_032873001.1	alanine racemase	-
KOM05_RS02505 (KOM05_02480)	489965..490246	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KOM05_RS02510 (KOM05_02485)	490252..490602	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KOM05_RS02515 (KOM05_02490)	490720..491541	+	822	WP_003156182.1	STAS domain-containing protein	-
KOM05_RS02520 (KOM05_02495)	491546..491911	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
KOM05_RS02525 (KOM05_02500)	491914..492315	+	402	WP_003156178.1	anti-sigma regulatory factor	-
KOM05_RS02530 (KOM05_02505)	492327..493334	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
KOM05_RS02535 (KOM05_02510)	493398..493727	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
KOM05_RS02540 (KOM05_02515)	493724..494206	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
KOM05_RS02545 (KOM05_02520)	494172..494960	+	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
KOM05_RS02550 (KOM05_02525)	494960..495562	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T205676 WP_003156187.1 NZ_CP076414:490252-490602 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T205676 NZ_CP101795:28131-28436 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT205676 WP_003156188.1 NZ_CP076414:489965-490246 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT205676 NZ_CP101795:27911-28129 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5