Detailed information of TA system TA205671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2540777..2541413
• Replicon: chromosome
• Accession: NZ_CP076409
• Organism: Bacillus sp. ZHX3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: ZHX2020_RS13505
• Protein ID: WP_003156187.1
• Coordinates: 2541063..2541413 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: ZHX2020_RS13500
• Protein ID: WP_003225183.1
• Coordinates: 2540777..2541058 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ZHX2020_RS13480 (ZHX2020_13410)	2537136..2537735	-	600	WP_014478896.1	rhomboid family intramembrane serine protease	-
ZHX2020_RS13485 (ZHX2020_13415)	2537830..2538195	+	366	WP_014478897.1	holo-ACP synthase	-
ZHX2020_RS13490 (ZHX2020_13420)	2538361..2539377	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
ZHX2020_RS13495 (ZHX2020_13425)	2539492..2540661	+	1170	WP_014478898.1	alanine racemase	-
ZHX2020_RS13500 (ZHX2020_13430)	2540777..2541058	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
ZHX2020_RS13505 (ZHX2020_13435)	2541063..2541413	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
ZHX2020_RS13510 (ZHX2020_13440)	2541529..2542353	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
ZHX2020_RS13515 (ZHX2020_13445)	2542358..2542723	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
ZHX2020_RS13520 (ZHX2020_13450)	2542727..2543128	+	402	WP_014478899.1	serine/threonine-protein kinase RsbT	-
ZHX2020_RS13525 (ZHX2020_13455)	2543140..2544147	+	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
ZHX2020_RS13530 (ZHX2020_13460)	2544216..2544545	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
ZHX2020_RS13535 (ZHX2020_13465)	2544542..2545024	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
ZHX2020_RS13540 (ZHX2020_13470)	2544990..2545778	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
ZHX2020_RS13545 (ZHX2020_13475)	2545778..2546377	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T205671 WP_003156187.1 NZ_CP076409:2541063-2541413 [Bacillus sp. ZHX3]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T205671 NZ_CP101793:c13040-12780 [Klebsiella pneumoniae]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGC
GAGGAAAAAAATGCTAACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT205671 WP_003225183.1 NZ_CP076409:2540777-2541058 [Bacillus sp. ZHX3]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT205671 NZ_CP101793:c12790-12539 [Klebsiella pneumoniae]
ATGCTAACTGAAAACACACCAGAAAACATTAAGCGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32