Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 481662..482299 Replicon chromosome
Accession NZ_CP076408
Organism Bacillus sp. JNUCC-22

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag JNUCC22_RS02460 Protein ID WP_003156187.1
Coordinates 481949..482299 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag JNUCC22_RS02455 Protein ID WP_003156188.1
Coordinates 481662..481943 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNUCC22_RS02435 (JNUCC22_02435) 478027..478626 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
JNUCC22_RS02440 (JNUCC22_02440) 478719..479084 + 366 WP_007410229.1 holo-ACP synthase -
JNUCC22_RS02445 (JNUCC22_02445) 479249..480256 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
JNUCC22_RS02450 (JNUCC22_02450) 480373..481542 + 1170 WP_216062106.1 alanine racemase -
JNUCC22_RS02455 (JNUCC22_02455) 481662..481943 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
JNUCC22_RS02460 (JNUCC22_02460) 481949..482299 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JNUCC22_RS02465 (JNUCC22_02465) 482417..483238 + 822 WP_003156182.1 STAS domain-containing protein -
JNUCC22_RS02470 (JNUCC22_02470) 483243..483608 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
JNUCC22_RS02475 (JNUCC22_02475) 483611..484012 + 402 WP_003156178.1 anti-sigma regulatory factor -
JNUCC22_RS02480 (JNUCC22_02480) 484024..485031 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
JNUCC22_RS02485 (JNUCC22_02485) 485095..485424 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
JNUCC22_RS02490 (JNUCC22_02490) 485421..485903 + 483 WP_003156173.1 anti-sigma B factor RsbW -
JNUCC22_RS02495 (JNUCC22_02495) 485869..486657 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
JNUCC22_RS02500 (JNUCC22_02500) 486657..487259 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T205669 WP_003156187.1 NZ_CP076408:481949-482299 [Bacillus sp. JNUCC-22]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T205669 NZ_CP101790:c4865966-4865049 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT205669 WP_003156188.1 NZ_CP076408:481662-481943 [Bacillus sp. JNUCC-22]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT205669 NZ_CP101790:c4866730-4865972 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References