Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 873361..874007 Replicon chromosome
Accession NZ_CP076406
Organism Providencia rettgeri strain PreM15793

Toxin (Protein)


Gene name Hha Uniprot ID B6XA97
Locus tag KOL65_RS03705 Protein ID WP_004905417.1
Coordinates 873361..873564 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID D4C184
Locus tag KOL65_RS03710 Protein ID WP_004905413.1
Coordinates 873639..874007 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOL65_RS03680 (KOL65_03710) 869436..869774 + 339 WP_004905421.1 P-II family nitrogen regulator -
KOL65_RS03685 (KOL65_03715) 869824..871071 + 1248 WP_228367686.1 ammonium transporter AmtB -
KOL65_RS03690 (KOL65_03720) 871210..872079 - 870 WP_109911901.1 acyl-CoA thioesterase II -
KOL65_RS03695 (KOL65_03725) 872319..872777 + 459 WP_140172116.1 YbaY family lipoprotein -
KOL65_RS03705 (KOL65_03735) 873361..873564 - 204 WP_004905417.1 HHA domain-containing protein Toxin
KOL65_RS03710 (KOL65_03740) 873639..874007 - 369 WP_004905413.1 Hha toxicity modulator TomB Antitoxin
KOL65_RS03715 (KOL65_03745) 874531..875901 - 1371 WP_136135575.1 murein transglycosylase D -
KOL65_RS03720 (KOL65_03750) 875983..876738 - 756 WP_140172117.1 hydroxyacylglutathione hydrolase -
KOL65_RS03725 (KOL65_03755) 876779..877513 + 735 WP_094962149.1 methyltransferase domain-containing protein -
KOL65_RS03730 (KOL65_03760) 877510..877980 - 471 WP_004905405.1 ribonuclease HI -
KOL65_RS03735 (KOL65_03765) 878035..878796 + 762 WP_140172118.1 DNA polymerase III subunit epsilon -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8061.34 Da        Isoelectric Point: 6.9770

>T205659 WP_004905417.1 NZ_CP076406:c873564-873361 [Providencia rettgeri]
MTKSDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Download         Length: 204 bp

>T205659 NZ_CP101789:c2121-1855 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14085.04 Da        Isoelectric Point: 4.4915

>AT205659 WP_004905413.1 NZ_CP076406:c874007-873639 [Providencia rettgeri]
MDEYSPKRHDIAELKYLCNSLNRDAILSLQKTNTHWINDLSSPQSAALNELIEHIAAFVWRFKIKYPKENLVISLIEEYL
DETYDLFGSPVITLSEIIDWEAMNHSLVAVLDDDLKCLTSKT

Download         Length: 369 bp

>AT205659 NZ_CP101789:c2329-2105 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A291E6Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A6G6Q6D9

References