Detailed information of TA system TA205656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2794625..2795160
• Replicon: chromosome
• Accession: NZ_CP076405
• Organism: Providencia rettgeri strain PreM15628

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: KOF27_RS12450
• Protein ID: WP_140170723.1
• Coordinates: 2794852..2795160 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: KOF27_RS12445
• Protein ID: WP_140170722.1
• Coordinates: 2794625..2794852 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOF27_RS12425 (KOF27_12465)	2789907..2790347	+	441	WP_110591516.1	flagellar export chaperone FlgN	-
KOF27_RS12430 (KOF27_12470)	2790534..2790965	+	432	WP_140170720.1	hypothetical protein	-
KOF27_RS12435 (KOF27_12475)	2791118..2793217	-	2100	WP_004255687.1	flagellar biosynthesis protein FlhA	-
KOF27_RS12440 (KOF27_12480)	2793210..2794361	-	1152	WP_140170721.1	flagellar biosynthesis protein FlhB	-
KOF27_RS12445 (KOF27_12485)	2794625..2794852	+	228	WP_140170722.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
KOF27_RS12450 (KOF27_12490)	2794852..2795160	+	309	WP_140170723.1	CcdB family protein	Toxin
KOF27_RS12455 (KOF27_12495)	2795176..2795733	+	558	WP_140170724.1	hypothetical protein	-
KOF27_RS12460 (KOF27_12500)	2795767..2796093	-	327	WP_282555854.1	helix-turn-helix transcriptional regulator	-
KOF27_RS12465 (KOF27_12505)	2796180..2796818	-	639	WP_094961165.1	protein phosphatase CheZ	-
KOF27_RS12470 (KOF27_12510)	2796843..2797235	-	393	WP_004255705.1	chemotaxis response regulator CheY	-
KOF27_RS12475 (KOF27_12515)	2797277..2798344	-	1068	WP_140170725.1	chemotaxis response regulator protein-glutamate methylesterase	-
KOF27_RS12480 (KOF27_12520)	2798344..2799180	-	837	WP_004255713.1	chemotaxis protein-glutamate O-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (5-75)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11770.87 Da
• Isoelectric Point: 8.0469

>T205656 WP_140170723.1 NZ_CP076405:2794852-2795160 [Providencia rettgeri]
MQYRLYQNRDDAIKYPYLLDIQSNIIDLLNTRLVIPLFDSRLVKKPLPARLNPQLVINGQVFILMTHQMACVPHSLLGKE
IVDLSSQRDTIKHAIDLLIDGF

Nucleotide

• Length: 309 bp

>T205656 NZ_CP101786:c75273-74803 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8660.87 Da
• Isoelectric Point: 8.9375

>AT205656 WP_140170722.1 NZ_CP076405:2794625-2794852 [Providencia rettgeri]
MNRTKKTVSVTVAPELYEQARRAKLNLSAILACALQAELKRLEAQKWKNDNAEGFNELNRINEEYGLLSDKYKDF

Nucleotide

• Length: 228 bp

>AT205656 NZ_CP101786:c75713-75270 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA