Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 873234..873880 Replicon chromosome
Accession NZ_CP076405
Organism Providencia rettgeri strain PreM15628

Toxin (Protein)


Gene name Hha Uniprot ID B6XA97
Locus tag KOF27_RS03705 Protein ID WP_004905417.1
Coordinates 873234..873437 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID D4C184
Locus tag KOF27_RS03710 Protein ID WP_004905413.1
Coordinates 873512..873880 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOF27_RS03680 (KOF27_03710) 869309..869647 + 339 WP_004905421.1 P-II family nitrogen regulator -
KOF27_RS03685 (KOF27_03715) 869697..870944 + 1248 WP_228367686.1 ammonium transporter AmtB -
KOF27_RS03690 (KOF27_03720) 871083..871952 - 870 WP_109911901.1 acyl-CoA thioesterase II -
KOF27_RS03695 (KOF27_03725) 872192..872650 + 459 WP_140172116.1 YbaY family lipoprotein -
KOF27_RS03705 (KOF27_03735) 873234..873437 - 204 WP_004905417.1 HHA domain-containing protein Toxin
KOF27_RS03710 (KOF27_03740) 873512..873880 - 369 WP_004905413.1 Hha toxicity modulator TomB Antitoxin
KOF27_RS03715 (KOF27_03745) 874404..875774 - 1371 WP_136135575.1 murein transglycosylase D -
KOF27_RS03720 (KOF27_03750) 875856..876611 - 756 WP_140172117.1 hydroxyacylglutathione hydrolase -
KOF27_RS03725 (KOF27_03755) 876652..877386 + 735 WP_094962149.1 methyltransferase domain-containing protein -
KOF27_RS03730 (KOF27_03760) 877383..877853 - 471 WP_004905405.1 ribonuclease HI -
KOF27_RS03735 (KOF27_03765) 877908..878669 + 762 WP_140172118.1 DNA polymerase III subunit epsilon -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8061.34 Da        Isoelectric Point: 6.9770

>T205654 WP_004905417.1 NZ_CP076405:c873437-873234 [Providencia rettgeri]
MTKSDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Download         Length: 204 bp

>T205654 NZ_CP101786:12535-12951 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14085.04 Da        Isoelectric Point: 4.4915

>AT205654 WP_004905413.1 NZ_CP076405:c873880-873512 [Providencia rettgeri]
MDEYSPKRHDIAELKYLCNSLNRDAILSLQKTNTHWINDLSSPQSAALNELIEHIAAFVWRFKIKYPKENLVISLIEEYL
DETYDLFGSPVITLSEIIDWEAMNHSLVAVLDDDLKCLTSKT

Download         Length: 369 bp

>AT205654 NZ_CP101786:12323-12538 [Klebsiella pneumoniae]
ATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAGCGAGCTGGAGATCGT
CCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCGAGAAGGCAGACCCGG
ACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A291E6Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A6G6Q6D9

References