Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4300254..4300751 Replicon chromosome
Accession NZ_CP076404
Organism Escherichia coli strain K-12 substr. AB1157

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KJY95_RS20755 Protein ID WP_001173343.1
Coordinates 4300578..4300751 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KJY95_RS20750 Protein ID WP_000398619.1
Coordinates 4300254..4300550 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJY95_RS20730 (4296985) 4296985..4297704 - 720 WP_000611288.1 two-component system response regulator DcuR -
KJY95_RS20735 (4297701) 4297701..4299332 - 1632 WP_001216477.1 sensor histidine kinase -
KJY95_RS20740 (4299513) 4299513..4299743 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KJY95_RS20745 (4299755) 4299755..4300027 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
KJY95_RS20750 (4300254) 4300254..4300550 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KJY95_RS20755 (4300578) 4300578..4300751 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KJY95_RS20760 (4300870) 4300870..4302387 - 1518 WP_001295074.1 lysine--tRNA ligase -
KJY95_RS20765 (4302624) 4302624..4304081 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T205647 WP_001173343.1 NZ_CP076404:4300578-4300751 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T205647 NZ_CP101784:c3341635-3341459 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGTATCAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT205647 WP_000398619.1 NZ_CP076404:4300254-4300550 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT205647 NZ_CP101784:c3341411-3341004 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCTTGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGTACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References