Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2987106..2987760 Replicon chromosome
Accession NZ_CP076404
Organism Escherichia coli strain K-12 substr. AB1157

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KJY95_RS14495 Protein ID WP_000244777.1
Coordinates 2987106..2987513 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KJY95_RS14500 Protein ID WP_000354046.1
Coordinates 2987494..2987760 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJY95_RS14475 (2983063) 2983063..2984796 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
KJY95_RS14480 (2984802) 2984802..2985512 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KJY95_RS14485 (2985537) 2985537..2986433 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KJY95_RS14490 (2986545) 2986545..2987066 + 522 WP_001055874.1 flavodoxin FldB -
KJY95_RS14495 (2987106) 2987106..2987513 - 408 WP_000244777.1 protein YgfX Toxin
KJY95_RS14500 (2987494) 2987494..2987760 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KJY95_RS14505 (2988003) 2988003..2988983 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KJY95_RS14510 (2989179) 2989179..2989838 - 660 WP_000250274.1 hemolysin III family protein -
KJY95_RS14515 (2990002) 2990002..2990313 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KJY95_RS14520 (2990358) 2990358..2991791 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KJY95_RS14525 (2991848) 2991848..2992591 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T205643 WP_000244777.1 NZ_CP076404:c2987513-2987106 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205643 NZ_CP101784:745320-745805 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205643 WP_000354046.1 NZ_CP076404:c2987760-2987494 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205643 NZ_CP101784:745030-745323 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References