Detailed information of TA system TA020564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 332862..333730
• Replicon: chromosome
• Accession: NC_002947
• Organism: Pseudomonas putida KT2440

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PP_RS01440
• Protein ID: WP_061405554.1
• Coordinates: 332862..333215 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q88R60
• Locus tag: PP_RS01445
• Protein ID: WP_004575922.1
• Coordinates: 333257..333730 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP_RS01420 (PP_0270)	327882..329249	-	1368	WP_010951639.1	HAMP domain-containing protein	-
PP_RS01425 (PP_0271)	329251..329970	-	720	WP_010951640.1	response regulator	-
PP_RS01430 (PP_0272)	330111..332408	+	2298	WP_010951641.1	TonB-dependent siderophore receptor	-
PP_RS01435 (PP_0273)	332474..332680	-	207	WP_014860378.1	DUF3079 domain-containing protein	-
PP_RS01440 (PP_5435)	332862..333215	+	354	WP_061405554.1	type II toxin-antitoxin system HigB family toxin	Toxin
PP_RS01445 (PP_0274)	333257..333730	+	474	WP_004575922.1	transcriptional regulator	Antitoxin
PP_RS01450 (PP_5436)	333735..333922	-	188	Protein_280	integrase	-
PP_RS01455 (PP_0275)	334150..334365	+	216	WP_010951644.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PP_RS01460 (PP_0276)	334362..334685	-	324	WP_010951645.1	helix-turn-helix domain-containing protein	-
PP_RS01470 (PP_0277)	335147..335395	-	249	WP_010951646.1	hypothetical protein	-
PP_RS01475 (PP_0278)	335916..336190	+	275	Protein_284	DUF3077 domain-containing protein	-
PP_RS01480 (PP_0279)	336545..337750	-	1206	WP_010951648.1	methyltransferase	-
PP_RS01485 (PP_0280)	337879..338568	-	690	WP_010951649.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13672.88 Da
• Isoelectric Point: 10.6182

>T20564 WP_061405554.1 NC_002947:332862-333215 [Pseudomonas putida KT2440]
MRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 354 bp

>T20564 NC_002947:332862-333215 [Pseudomonas putida KT2440]
ATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGATTGAAGTCCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCT
GACGACCTTCGACAGGGCGACGCTGCGGTTCGAATCTTTTGAACAGTTGAGGCAGACGTGGGCCACTGTGTCTGGCTGGA
ATGTTGATCGCATCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAG
AAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGA
GTACGACAAATGGTGCAGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17229.67 Da
• Isoelectric Point: 4.4982

>AT20564 WP_004575922.1 NC_002947:333257-333730 [Pseudomonas putida KT2440]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT20564 NC_002947:333257-333730 [Pseudomonas putida KT2440]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CCTTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGATAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CBJ4