Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2035608..2036439 Replicon chromosome
Accession NZ_CP076404
Organism Escherichia coli strain K-12 substr. AB1157

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag KJY95_RS10050 Protein ID WP_000854814.1
Coordinates 2036065..2036439 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag KJY95_RS10045 Protein ID WP_001285584.1
Coordinates 2035608..2035976 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJY95_RS10030 (2033275) 2033275..2034807 + 1533 WP_001350525.1 protein YeeR -
KJY95_RS10035 (2034804) 2034804..2035250 + 447 WP_000187523.1 RadC family protein -
KJY95_RS10040 (2035313) 2035313..2035534 + 222 WP_000692323.1 DUF987 domain-containing protein -
KJY95_RS10045 (2035608) 2035608..2035976 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
KJY95_RS10050 (2036065) 2036065..2036439 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KJY95_RS10055 (2036436) 2036436..2036630 + 195 WP_000988600.1 DUF5983 family protein -
KJY95_RS10060 (2036676) 2036676..2036756 + 81 Protein_1967 hypothetical protein -
KJY95_RS10065 (2037045) 2037045..2037173 - 129 Protein_1968 transposase domain-containing protein -
KJY95_RS10070 (2037293) 2037293..2037427 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
KJY95_RS10075 (2037528) 2037528..2037857 - 330 WP_000450409.1 DUF496 family protein -
KJY95_RS10080 (2038029) 2038029..2039087 - 1059 WP_001200891.1 FUSC family protein -
KJY95_RS10085 (2039285) 2039285..2039758 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
KJY95_RS10090 (2039877) 2039877..2041043 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T205638 WP_000854814.1 NZ_CP076404:2036065-2036439 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T205638 NZ_CP101781:c8087-7773 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT205638 WP_001285584.1 NZ_CP076404:2035608-2035976 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT205638 NZ_CP101781:c8353-8075 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References