Detailed information of TA system TA205603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3530525..3531150
• Replicon: chromosome
• Accession: NZ_CP076386
• Organism: Dickeya dadantii strain S3-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E0SD87
• Locus tag: KNV89_RS15670
• Protein ID: WP_009114000.1
• Coordinates: 3530525..3530728 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: KNV89_RS15675
• Protein ID: WP_216282078.1
• Coordinates: 3530782..3531150 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNV89_RS15640 (KNV89_15640)	3526183..3526521	+	339	WP_002208627.1	P-II family nitrogen regulator	-
KNV89_RS15645 (KNV89_15645)	3526564..3527856	+	1293	WP_216282074.1	ammonium transporter AmtB	-
KNV89_RS15650 (KNV89_15650)	3527951..3528814	-	864	WP_216282075.1	acyl-CoA thioesterase II	-
KNV89_RS15655 (KNV89_15655)	3529049..3529606	+	558	WP_216282076.1	YbaY family lipoprotein	-
KNV89_RS15660 (KNV89_15660)	3529643..3529966	-	324	WP_216282077.1	MGMT family protein	-
KNV89_RS15670 (KNV89_15670)	3530525..3530728	-	204	WP_009114000.1	HHA domain-containing protein	Toxin
KNV89_RS15675 (KNV89_15675)	3530782..3531150	-	369	WP_216282078.1	Hha toxicity modulator TomB	Antitoxin
KNV89_RS15680 (KNV89_15680)	3531657..3531800	-	144	WP_216282079.1	type B 50S ribosomal protein L36	-
KNV89_RS15685 (KNV89_15685)	3531816..3532067	-	252	WP_216282080.1	type B 50S ribosomal protein L31	-
KNV89_RS15690 (KNV89_15690)	3532248..3535394	-	3147	WP_216282081.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8133.51 Da
• Isoelectric Point: 8.8500

>T205603 WP_009114000.1 NZ_CP076386:c3530728-3530525 [Dickeya dadantii]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Nucleotide

• Length: 204 bp

>T205603 NZ_CP101770:329318-329686 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14240.10 Da
• Isoelectric Point: 5.1811

>AT205603 WP_216282078.1 NZ_CP076386:c3531150-3530782 [Dickeya dadantii]
MDEYTPQHYDIAQLRFLCENLHDESIATLGDSSRGWVNDPTSAVNLQLNELIEHIAAFVVTYKIKYPHEAALCERVEKYL
DDTYILFSNYGINDAELRKWQKSRSQLFRMFSEKSICTVVKT

Nucleotide

• Length: 369 bp

>AT205603 NZ_CP101770:329100-329321 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A1X3RNQ0