Detailed information of TA system TA205597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2253460..2254124
• Replicon: chromosome
• Accession: NZ_CP076382
• Organism: Mycobacterium intracellulare ATCC 13950

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IUS5
• Locus tag: KN251_RS10730
• Protein ID: WP_014379897.1
• Coordinates: 2253717..2254124 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KN251_RS10725
• Protein ID: WP_009954496.1
• Coordinates: 2253460..2253720 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN251_RS10705 (KN251_10655)	2249977..2250468	-	492	WP_014379893.1	DUF1707 domain-containing protein	-
KN251_RS10710 (KN251_10660)	2250538..2251134	+	597	WP_009954492.1	TetR/AcrR family transcriptional regulator	-
KN251_RS10715 (KN251_10665)	2251139..2252407	-	1269	WP_014379895.1	cytochrome P450	-
KN251_RS10720 (KN251_10670)	2252565..2253017	+	453	WP_014379896.1	VOC family protein	-
KN251_RS10725 (KN251_10675)	2253460..2253720	+	261	WP_009954496.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
KN251_RS10730 (KN251_10680)	2253717..2254124	+	408	WP_014379897.1	type II toxin-antitoxin system VapC family toxin	Toxin
KN251_RS10735 (KN251_10685)	2254302..2257403	+	3102	WP_014379898.1	DEAD/DEAH box helicase	-
KN251_RS10740 (KN251_10690)	2257464..2259029	-	1566	WP_014379899.1	serine/threonine-protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14307.31 Da
• Isoelectric Point: 4.2772

>T205597 WP_014379897.1 NZ_CP076382:2253717-2254124 [Mycobacterium intracellulare ATCC 13950]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPIDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T205597 NZ_CP101765:193147-193458 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT205597 WP_009954496.1 NZ_CP076382:2253460-2253720 [Mycobacterium intracellulare ATCC 13950]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT205597 NZ_CP101765:193455-193874 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7R7MTD0