Detailed information of TA system TA205595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 887542..888195
• Replicon: chromosome
• Accession: NZ_CP076382
• Organism: Mycobacterium intracellulare ATCC 13950

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: KN251_RS04545
• Protein ID: WP_014379252.1
• Coordinates: 887734..888195 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: KN251_RS04540
• Protein ID: WP_009957321.1
• Coordinates: 887542..887730 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN251_RS04530 (KN251_04510)	883515..885092	+	1578	WP_014379250.1	serine hydrolase	-
KN251_RS04535 (KN251_04515)	885089..887476	+	2388	WP_014379251.1	cation-translocating P-type ATPase	-
KN251_RS04540 (KN251_04520)	887542..887730	+	189	WP_009957321.1	antitoxin	Antitoxin
KN251_RS04545 (KN251_04525)	887734..888195	+	462	WP_014379252.1	SRPBCC family protein	Toxin
KN251_RS04550 (KN251_04530)	888279..889049	+	771	WP_014379253.1	VOC family protein	-
KN251_RS04555 (KN251_04535)	889123..889557	+	435	WP_008253914.1	hypothetical protein	-
KN251_RS04560 (KN251_04540)	889562..890050	-	489	WP_036458243.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
KN251_RS04565 (KN251_04545)	890050..891576	-	1527	WP_014379255.1	carotenoid oxygenase family protein	-
KN251_RS04570 (KN251_04550)	891578..892798	-	1221	WP_014379256.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16609.18 Da
• Isoelectric Point: 8.5617

>T205595 WP_014379252.1 NZ_CP076382:887734-888195 [Mycobacterium intracellulare ATCC 13950]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVAVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T205595 NZ_CP101764:c5325868-5325485 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT205595 WP_009957321.1 NZ_CP076382:887542-887730 [Mycobacterium intracellulare ATCC 13950]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT205595 NZ_CP101764:c5326110-5325868 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: X8A187