Detailed information of TA system TA205594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 2597114..2598106
• Replicon: chromosome
• Accession: NZ_CP076381
• Organism: Mycobacterium intracellulare strain S1-36A

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: KN247_RS12185
• Protein ID: WP_038536181.1
• Coordinates: 2597528..2598106 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: X8A634
• Locus tag: KN247_RS12180
• Protein ID: WP_038536179.1
• Coordinates: 2597114..2597503 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN247_RS12155 (KN247_12090)	2592565..2594568	+	2004	WP_095562692.1	phosphotransferase	-
KN247_RS12160 (KN247_12095)	2594565..2595065	+	501	WP_038536171.1	gluconokinase	-
KN247_RS12165 (KN247_12100)	2595136..2595747	-	612	WP_095562693.1	TetR/AcrR family transcriptional regulator	-
KN247_RS12170 (KN247_12105)	2595783..2596742	+	960	WP_225331100.1	SAM-dependent methyltransferase	-
KN247_RS12175 (KN247_12110)	2596770..2597057	+	288	WP_014941865.1	hypothetical protein	-
KN247_RS12180 (KN247_12115)	2597114..2597503	+	390	WP_038536179.1	hypothetical protein	Antitoxin
KN247_RS12185 (KN247_12120)	2597528..2598106	+	579	WP_038536181.1	zeta toxin family protein	Toxin
KN247_RS12190 (KN247_12125)	2598126..2599193	-	1068	WP_038536184.1	DoxX family protein	-
KN247_RS12195 (KN247_12130)	2599289..2599849	+	561	WP_042911761.1	pyrazinamidase PncA	-
KN247_RS12200 (KN247_12135)	2599849..2600649	+	801	WP_009956375.1	nuclear transport factor 2 family protein	-
KN247_RS12205 (KN247_12140)	2600661..2601011	-	351	WP_020188920.1	DoxX family protein	-
KN247_RS12210 (KN247_12145)	2601177..2602148	+	972	WP_014382494.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-137)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21103.22 Da
• Isoelectric Point: 11.0233

>T205594 WP_038536181.1 NZ_CP076381:2597528-2598106 [Mycobacterium intracellulare]
MQRLDLVVGPNGAGKSTFIPFTLAPLLPGSPVVNADEIARQRWPGESAGRAYDAAQIAAATRAKLIDLGRSFIAETVFSH
PSKLDLIRTAHRAGFTVVLHAMLIPEDLAVERVRHRVRAGGHDVPETKVRERHRRLWTPVTDAIALSDVATVYDNSRLRG
PRIVARLSGGMTIGSPDWPAWAPEILRSRWPD

Nucleotide

• Length: 579 bp

>T205594 NZ_CP101764:c5001151-5000234 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13507.88 Da
• Isoelectric Point: 5.5186

>AT205594 WP_038536179.1 NZ_CP076381:2597114-2597503 [Mycobacterium intracellulare]
MADAPDRVTRVASDLMDSAASEGARQSRSAKQQLDHWARVGRAVSSQHTASRRRVEAALAGHLATGELTVEEGVVFNAEI
SAAIDESLVHADYGATLAGHGVTTVALNEDGDIVEHRPDGAAVVLARGR

Nucleotide

• Length: 390 bp

>AT205594 NZ_CP101764:c5001915-5001157 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: X8A634