Detailed information of TA system TA205593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2253077..2253741
• Replicon: chromosome
• Accession: NZ_CP076381
• Organism: Mycobacterium intracellulare strain S1-36A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9WD27
• Locus tag: KN247_RS10730
• Protein ID: WP_009954498.1
• Coordinates: 2253334..2253741 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KN247_RS10725
• Protein ID: WP_009954496.1
• Coordinates: 2253077..2253337 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN247_RS10700 (KN247_10645)	2249585..2250076	-	492	WP_201368376.1	DUF1707 domain-containing protein	-
KN247_RS10705 (KN247_10650)	2250146..2250742	+	597	WP_201368381.1	TetR/AcrR family transcriptional regulator	-
KN247_RS10710 (KN247_10655)	2250747..2252015	-	1269	WP_201368375.1	cytochrome P450	-
KN247_RS10715 (KN247_10660)	2252176..2252628	+	453	WP_029384640.1	VOC family protein	-
KN247_RS10720 (KN247_10665)	2252665..2252904	+	240	WP_009954495.1	hypothetical protein	-
KN247_RS10725 (KN247_10670)	2253077..2253337	+	261	WP_009954496.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
KN247_RS10730 (KN247_10675)	2253334..2253741	+	408	WP_009954498.1	type II toxin-antitoxin system VapC family toxin	Toxin
KN247_RS10735 (KN247_10680)	2253919..2257020	+	3102	WP_009954499.1	DEAD/DEAH box helicase	-
KN247_RS10740 (KN247_10685)	2257082..2258647	-	1566	WP_009954500.1	protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14293.28 Da
• Isoelectric Point: 4.2772

>T205593 WP_009954498.1 NZ_CP076381:2253334-2253741 [Mycobacterium intracellulare]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPVDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T205593 NZ_CP101764:c4844334-4844050 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT205593 WP_009954496.1 NZ_CP076381:2253077-2253337 [Mycobacterium intracellulare]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT205593 NZ_CP101764:c4844566-4844324 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: J9WD27