Detailed information of TA system TA205591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 886690..887343
• Replicon: chromosome
• Accession: NZ_CP076381
• Organism: Mycobacterium intracellulare strain S1-36A

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: KN247_RS04545
• Protein ID: WP_014379252.1
• Coordinates: 886882..887343 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: KN247_RS04540
• Protein ID: WP_009957321.1
• Coordinates: 886690..886878 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN247_RS04530 (KN247_04505)	882663..884240	+	1578	WP_014379250.1	serine hydrolase	-
KN247_RS04535 (KN247_04510)	884237..886624	+	2388	WP_014379251.1	cation-translocating P-type ATPase	-
KN247_RS04540 (KN247_04515)	886690..886878	+	189	WP_009957321.1	antitoxin	Antitoxin
KN247_RS04545 (KN247_04520)	886882..887343	+	462	WP_014379252.1	SRPBCC family protein	Toxin
KN247_RS04550 (KN247_04525)	887427..888197	+	771	WP_014379253.1	VOC family protein	-
KN247_RS04555 (KN247_04530)	888271..888705	+	435	WP_008253914.1	hypothetical protein	-
KN247_RS04560 (KN247_04535)	888710..889198	-	489	WP_036458243.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
KN247_RS04565 (KN247_04540)	889198..890724	-	1527	WP_038535114.1	carotenoid oxygenase family protein	-
KN247_RS04570 (KN247_04545)	890726..891946	-	1221	WP_036428523.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16609.18 Da
• Isoelectric Point: 8.5617

>T205591 WP_014379252.1 NZ_CP076381:886882-887343 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVAVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T205591 NZ_CP101764:4124752-4124970 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT205591 WP_009957321.1 NZ_CP076381:886690-886878 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT205591 NZ_CP101764:4124351-4124725 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: X8A187