Detailed information of TA system TA205590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/ParD(antitoxin)
• Location: 2618436..2619428
• Replicon: chromosome
• Accession: NZ_CP076380
• Organism: Mycobacterium intracellulare strain S1-32

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: KN246_RS12305
• Protein ID: WP_009956379.1
• Coordinates: 2618850..2619428 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: A0A7I7TS83
• Locus tag: KN246_RS12300
• Protein ID: WP_009956380.1
• Coordinates: 2618436..2618825 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN246_RS12275 (KN246_12210)	2613888..2615891	+	2004	WP_009956386.1	phosphotransferase	-
KN246_RS12280 (KN246_12215)	2615888..2616433	+	546	WP_044059213.1	gluconokinase	-
KN246_RS12285 (KN246_12220)	2616457..2617068	-	612	WP_009956383.1	TetR/AcrR family transcriptional regulator	-
KN246_RS12290 (KN246_12225)	2617104..2618063	+	960	WP_225331100.1	SAM-dependent methyltransferase	-
KN246_RS12295 (KN246_12230)	2618091..2618378	+	288	WP_225332075.1	hypothetical protein	-
KN246_RS12300 (KN246_12235)	2618436..2618825	+	390	WP_009956380.1	hypothetical protein	Antitoxin
KN246_RS12305 (KN246_12240)	2618850..2619428	+	579	WP_009956379.1	zeta toxin family protein	Toxin
KN246_RS12310 (KN246_12245)	2619448..2620515	-	1068	WP_036397470.1	DoxX family protein	-
KN246_RS12315 (KN246_12250)	2620611..2621171	+	561	WP_029385272.1	pyrazinamidase PncA	-
KN246_RS12320 (KN246_12255)	2621171..2621971	+	801	WP_009956375.1	nuclear transport factor 2 family protein	-
KN246_RS12325 (KN246_12260)	2621983..2622333	-	351	WP_009956374.1	DoxX family protein	-
KN246_RS12330 (KN246_12265)	2622499..2623470	+	972	WP_029385267.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 20962.05 Da
• Isoelectric Point: 11.0233

>T205590 WP_009956379.1 NZ_CP076380:2618850-2619428 [Mycobacterium intracellulare]
MQRLDLVVGPNGAGKSTFIAFTLAPLLPGSPVVNADEIARQRWPGEAAGRAYDAAQIAAATRAKLIDLGRSFIAETVFSH
PSKLDLVRTAHRAGFTAVLHAMLIPEDLAVERVRHRVRAGGHDVPETKVRERHRRLWTPVTDAIALSDVATVYDSGRLRG
PRIVARLSGGMTIGSPDWPAWAPEILRSRWPD

Nucleotide

• Length: 579 bp

>T205590 NZ_CP101764:c3302106-3301930 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGTATCAAATAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13512.89 Da
• Isoelectric Point: 5.1828

>AT205590 WP_009956380.1 NZ_CP076380:2618436-2618825 [Mycobacterium intracellulare]
MADAPDRVTRVASDLMDSAASEGARQSRSAKQQLDHWARVGRAVSSQHTASRRRVEAALAGQLATGELTVEEGVVFNAEI
SAAIEESLVHADYGATLAGHGVTTVALNEDGDIVEHRPDGAAVVLARGR

Nucleotide

• Length: 390 bp

>AT205590 NZ_CP101764:c3301882-3301475 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCTTGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGTACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7TS83