Detailed information of TA system TA205587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 888451..889104
• Replicon: chromosome
• Accession: NZ_CP076380
• Organism: Mycobacterium intracellulare strain S1-32

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: KN246_RS04560
• Protein ID: WP_008253911.1
• Coordinates: 888643..889104 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: KN246_RS04555
• Protein ID: WP_009957321.1
• Coordinates: 888451..888639 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN246_RS04545 (KN246_04520)	884424..886001	+	1578	WP_009957319.1	serine hydrolase	-
KN246_RS04550 (KN246_04525)	885998..888385	+	2388	WP_009957320.1	cation-translocating P-type ATPase	-
KN246_RS04555 (KN246_04530)	888451..888639	+	189	WP_009957321.1	antitoxin	Antitoxin
KN246_RS04560 (KN246_04535)	888643..889104	+	462	WP_008253911.1	SRPBCC family protein	Toxin
KN246_RS04565 (KN246_04540)	889189..889959	+	771	WP_009957322.1	VOC family protein	-
KN246_RS04570 (KN246_04545)	890033..890467	+	435	WP_008253914.1	hypothetical protein	-
KN246_RS04575 (KN246_04550)	890472..890960	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
KN246_RS04580 (KN246_04555)	890960..892486	-	1527	WP_014381862.1	carotenoid oxygenase family protein	-
KN246_RS04585 (KN246_04560)	892488..893708	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T205587 WP_008253911.1 NZ_CP076380:888643-889104 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T205587 NZ_CP101764:883759-884166 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT205587 WP_009957321.1 NZ_CP076380:888451-888639 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT205587 NZ_CP101764:883512-883778 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: X8A187