Detailed information of TA system TA205584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 993115..993768
• Replicon: chromosome
• Accession: NZ_CP076379
• Organism: Mycobacterium intracellulare strain S2-11

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: KN252_RS05025
• Protein ID: WP_008253911.1
• Coordinates: 993307..993768 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I2A997
• Locus tag: KN252_RS05020
• Protein ID: WP_008253910.1
• Coordinates: 993115..993303 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN252_RS05010 (KN252_04995)	989088..990665	+	1578	WP_014381859.1	serine hydrolase	-
KN252_RS05015 (KN252_05000)	990662..993049	+	2388	WP_095762870.1	cation-translocating P-type ATPase	-
KN252_RS05020 (KN252_05005)	993115..993303	+	189	WP_008253910.1	antitoxin	Antitoxin
KN252_RS05025 (KN252_05010)	993307..993768	+	462	WP_008253911.1	SRPBCC family protein	Toxin
KN252_RS05030 (KN252_05015)	993853..994623	+	771	WP_008253913.1	VOC family protein	-
KN252_RS05035 (KN252_05020)	994697..995131	+	435	WP_008253914.1	hypothetical protein	-
KN252_RS05040 (KN252_05025)	995136..995624	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
KN252_RS05045 (KN252_05030)	995624..997150	-	1527	WP_014381862.1	carotenoid oxygenase family protein	-
KN252_RS05050 (KN252_05035)	997152..998372	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T205584 WP_008253911.1 NZ_CP076379:993307-993768 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T205584 NZ_CP101764:328118-328486 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6797.44 Da
• Isoelectric Point: 4.4702

>AT205584 WP_008253910.1 NZ_CP076379:993115-993303 [Mycobacterium intracellulare]
MGFLDKAKDLLWQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT205584 NZ_CP101764:327900-328121 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7TPT2