Detailed information of TA system TA205583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4574874..4575527
• Replicon: chromosome
• Accession: NZ_CP076378
• Organism: Mycobacterium intracellulare strain S2-8

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: KN249_RS21400
• Protein ID: WP_008253911.1
• Coordinates: 4574874..4575335 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: KN249_RS21405
• Protein ID: WP_009957321.1
• Coordinates: 4575339..4575527 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN249_RS21375 (KN249_21315)	4570270..4571490	+	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-
KN249_RS21380 (KN249_21320)	4571492..4573018	+	1527	WP_014381862.1	carotenoid oxygenase family protein	-
KN249_RS21385 (KN249_21325)	4573018..4573506	+	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
KN249_RS21390 (KN249_21330)	4573511..4573945	-	435	WP_008253914.1	hypothetical protein	-
KN249_RS21395 (KN249_21335)	4574019..4574789	-	771	WP_009957322.1	VOC family protein	-
KN249_RS21400 (KN249_21340)	4574874..4575335	-	462	WP_008253911.1	SRPBCC family protein	Toxin
KN249_RS21405 (KN249_21345)	4575339..4575527	-	189	WP_009957321.1	antitoxin	Antitoxin
KN249_RS21410 (KN249_21350)	4575593..4577980	-	2388	WP_009957320.1	cation-translocating P-type ATPase	-
KN249_RS21415 (KN249_21355)	4577977..4579554	-	1578	WP_009957319.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T205583 WP_008253911.1 NZ_CP076378:c4575335-4574874 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T205583 NZ_CP101752:c6348153-6347788 [Pseudomonas citronellolis]
ATGGTGAAGGCGCTGTTCGACACCAACATCCTCATCGACTACCTCAACGGCCACGAACAGGCCCGCGACGAGCTGCGCCG
CTACGATGACCCCGCCATCAGCATCGTCACCTGGATGGAGGTGATGATCGGCGCCACGCCCGCCACCGCCGCGGCCACCC
GCGCCTTCCTCGACAGCTTCGCCCTGGTGCCGCTCGACGCGTCCGTCGCCGAGCGCGCCGTGGCGGTGCGCCAGGCGCTG
CGGGTGAAGCTGCCGGACGCCATCATCAAGGCCAGCGCCGAGGTGCAGGGGCGCCTGCTGGTCACCCGCAACACCAGGGA
CTTCCCCGTCGACGACGCCGGCGTGCGCCTGCCCTACCGGCTCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT205583 WP_009957321.1 NZ_CP076378:c4575527-4575339 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT205583 NZ_CP101752:c6348362-6348147 [Pseudomonas citronellolis]
ATGCGTACCCTGGTCGACATCCCTGCCGAACACCTCGAAGCGCTCAATGCCCTGGGCGAGCAACAGCAGCAATCGCGCGC
CGCGCTGATCCGCGAGGCGGTCGCCGAGTACCTGGTCCGGCACCGCCGGCAGGATGCCGAGGCCGCCTTCGGCCTGTGGA
ACGGCAGCGCCGGCGACGGCCTGGCCTACCAGGAGAAGGTGCGCGAGGAATGGTGA

Structures

Antitoxin

• AlphaFold DB: X8A187