Detailed information of TA system TA205582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4324875..4325439
• Replicon: chromosome
• Accession: NZ_CP076378
• Organism: Mycobacterium intracellulare strain S2-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IVI4
• Locus tag: KN249_RS20250
• Protein ID: WP_009953437.1
• Coordinates: 4324875..4325246 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KN249_RS20255
• Protein ID: WP_026071432.1
• Coordinates: 4325233..4325439 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN249_RS20210 (KN249_20155)	4320331..4320540	-	210	WP_014381976.1	hypothetical protein	-
KN249_RS20215 (KN249_20160)	4320687..4321370	+	684	WP_014384004.1	hypothetical protein	-
KN249_RS20220 (KN249_20165)	4321419..4321742	-	324	WP_009953431.1	putative quinol monooxygenase	-
KN249_RS20225	4322309..4322737	-	429	Protein_3998	hypothetical protein	-
KN249_RS20230 (KN249_20175)	4322862..4323221	-	360	WP_179293417.1	hypothetical protein	-
KN249_RS20235 (KN249_20180)	4323307..4323690	-	384	WP_026071433.1	hypothetical protein	-
KN249_RS20240 (KN249_20185)	4323789..4324187	-	399	WP_009953435.1	VOC family protein	-
KN249_RS20245 (KN249_20190)	4324266..4324817	-	552	WP_009953436.1	VOC family protein	-
KN249_RS20250 (KN249_20195)	4324875..4325246	-	372	WP_009953437.1	type II toxin-antitoxin system VapC family toxin	Toxin
KN249_RS20255 (KN249_20200)	4325233..4325439	-	207	WP_026071432.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KN249_RS20260 (KN249_20205)	4325678..4326340	+	663	WP_014379386.1	methyltransferase domain-containing protein	-
KN249_RS20265 (KN249_20210)	4326420..4327493	-	1074	WP_009953440.1	redox-regulated ATPase YchF	-
KN249_RS20270 (KN249_20215)	4327635..4328774	+	1140	WP_179293415.1	hypothetical protein	-
KN249_RS20275 (KN249_20220)	4328820..4329818	-	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13868.20 Da
• Isoelectric Point: 6.8398

>T205582 WP_009953437.1 NZ_CP076378:c4325246-4324875 [Mycobacterium intracellulare]
VILVDTSIWIDHLHVAEQRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSHLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACKEVGVAIVDES

Nucleotide

• Length: 372 bp

>T205582 NZ_CP101752:6324708-6325118 [Pseudomonas citronellolis]
GTGAAATACCTGCTCGACACCAACATCCTCATCTACCTGCTGAAGAACCGCCCGGAATCCGTGGCCCGACGGGTCAACGC
CCTGCCCGCCGACGCCGGCCTGTGCATGTCCTTCTTCACCTACGCAGAACTGCTCAAGGGCGCCGAACGCAGCACCCGGC
GCAGCGAGGTCCTGCGCCAGCTGGAGCGCCTGACGCGCCAGGTGCCGGTGGTCTACGACGGTACCCCACGCCTGTGCGAG
CACTACGCGACGCAGTTCACCCGCCTCAAGCTGGCCGGCACGCCGATCGGCGCCAACGACCTATGGATCGCCTGCCACGC
CCTGGCGCTGGAGGCGACCCTGGTGACGCACAACACCCGCGAGTTCGAACGCATCGACGGGCTGTGCCTGGAAGATTGGG
CTGCCGAGTAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7250.31 Da
• Isoelectric Point: 11.9169

>AT205582 WP_026071432.1 NZ_CP076378:c4325439-4325233 [Mycobacterium intracellulare]
MRTTVTIDDALLAQAAELTGVTESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT205582 NZ_CP101752:6324451-6324711 [Pseudomonas citronellolis]
ATGTCCTCGCCCACACTCCCCAGCCGCGTCTTCATGAACGGCAACAGCCAGGCGGTGCGCATTCCCCAGGAGTTCCGCCT
GGACGCCAGCCGCGTGGAGATCCGCCGTCAGGCCAATGGCGACCTGGTCATCCACCCATTGCCCGAGGACCGCGGCGAGG
CGTTGCTGCAGGCGCTGTCCGGCTTCGACGAGGCGTTCATCGAAGCGCTGGAGGAAGGGCAGCGCGAACAGCCGCCGATG
CAGGAGCGGGAAGAGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7R7MQX9