Detailed information of TA system TA020556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3144066..3144753
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: BQ2027_RS14775
• Protein ID: WP_003414624.1
• Coordinates: 3144310..3144753 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: BQ2027_RS14770
• Protein ID: WP_003414620.1
• Coordinates: 3144066..3144323 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS14750 (BQ2027_MB2892C)	3139386..3140240	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
BQ2027_RS14755 (BQ2027_MB2893C)	3140296..3141459	-	1164	WP_010950793.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
BQ2027_RS14760 (BQ2027_MB2894C)	3141476..3142690	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
BQ2027_RS14765 (BQ2027_MB2895C)	3142698..3143939	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
BQ2027_RS14770 (BQ2027_MB2896)	3144066..3144323	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
BQ2027_RS14775 (BQ2027_MB2897)	3144310..3144753	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
BQ2027_RS14780 (BQ2027_MB2898)	3144833..3145495	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
BQ2027_RS14785	3145591..3145782	+	192	WP_023349587.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
BQ2027_RS14790 (BQ2027_MB2899)	3146114..3147862	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
BQ2027_RS14795 (BQ2027_MB2900)	3147958..3148539	+	582	WP_003414644.1	fasciclin domain-containing protein	-
BQ2027_RS14800 (BQ2027_MB2901)	3148639..3148905	+	267	WP_031652375.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T20556 WP_003414624.1 NC_002945:3144310-3144753 [Mycobacterium tuberculosis variant bovis AF2122/97]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T20556 NC_002945:3144310-3144753 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT20556 WP_003414620.1 NC_002945:3144066-3144323 [Mycobacterium tuberculosis variant bovis AF2122/97]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT20556 NC_002945:3144066-3144323 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0